Effects of celastrol on the proliferation and apoptosis of human leukemia cells HL-60 and Jurkat
10.11665/j.issn.1000-5048.20150113
- VernacularTitle:雷公藤红素对白血病HL-60和Jurkat细胞增殖及凋亡的影响
- Author:
Xiaoling ZHANG
1
;
Aiping LI
;
Fei HUAN
;
Heng QIN
;
Jin′ao DUAN
Author Information
1. 南京医科大学公共卫生学院
- Publication Type:Journal Article
- Keywords:
celastrol;
HL-60 cell;
Jurkat cell;
apoptosis
- From:
Journal of China Pharmaceutical University
2015;46(1):89-93
- CountryChina
- Language:Chinese
-
Abstract:
To determine the effects of celastrol on the proliferation and apoptosis of cell lines HL-60 and Jurkat in human leukemia. Human leukemia cell lines were treated with celastrol at different concentrations. The inhibitory rate of cell proliferation was detected by MTT assay; the apoptosis rate was detected by AnnexinV-FITC/PI double staining; cell cycle was observed by PI staining; cell morphology was observed by transmission electron microscope(TEM). Cell proliferation was inhibited by celastrol, with IC50 of(0. 46±1. 05)μmol/L and(0. 88±1. 13)μmol/L at 24 h. Celastrol induced cell apoptosis in a dose-dependent manner. The cell cycle distribution of G1 phase rate increased, S phase rate decreased(P< 0. 05)with typical cell apoptosis-induced morphological changes. Results showed that celastrol could significantly inhibit cell proliferation and induce apoptosis in human leukemia cell lines of HL-60 and Jurkat.
