Determination of 5-fluorouracil in serum by direct protein precipitation-ultra performance liquid chromatography
10.11665/j.issn.1000-5048.20150111
- VernacularTitle:直接蛋白沉淀-超高效液相色谱法测定氟尿嘧啶血药浓度
- Author:
Wenbo YUAN
1
;
Yongjuan DING
;
Jingjing XU
Author Information
1. 无锡市第四人民医院
- Publication Type:Journal Article
- Keywords:
protein precipitation;
ultra performance liquid chromatography;
5-fluorouracil;
determination
- From:
Journal of China Pharmaceutical University
2015;46(1):81-84
- CountryChina
- Language:Chinese
-
Abstract:
A method based on direct protein precipitation-ultra performance liquid chromatography was established for the determination of trace 5-fluorouracil in serum. The samples were pretreated with 6% perchloric acid as the precipitant and 5-chlorouracil as the internal standard, and the supernatant separated after vortex mixing and high speed centrifugation was used as the test sample. The separation was carried out with an Acquity HSS T3 column and the mobile phase consisting of water and acetonitrile, and the flow rate was 0. 2 mL/min; the injection volume was 5 μL, and the column temperature was 20 °C. The key parameters of this method such as specificity, accuracy, precision and linearity, were validated. Compared with other methods, this method can complete the analysis with significantly reduced time and cost. The established method is useful for therapeutic drug monitoring of 5-fluorouracil plasma concentration and it has been successfully applied in clinical cases.
