Determination of the related substances of tenofovir by RP-HPLC
10.11665/j.issn.1000-5048.20150110
- VernacularTitle:RP-HPLC测定替诺福韦及其有关物质
- Author:
Chao LIU
1
;
Xing ZHONG
;
Yanqiong XI
;
Yinxia TANG
;
Li JIANG
;
Fujun WANG
;
Tong PU
;
Su ZENG
Author Information
1. 浙江大学药学院；浙江车头制药股份有限公司；浙江省抗病毒药物工程技术研究中心
- Publication Type:Journal Article
- Keywords:
tenofovir;
RP-HPLC;
related substances;
impurities
- From:
Journal of China Pharmaceutical University
2015;46(1):78-80
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the related substances of tenofovir, 3 related substances were synthesized. RP-HPLC analytical method was developed using Agilent ZORBAX SB-C18 column. The mobile phase composed of methanol and 0. 02 mol/L potassium dihydrogen phosphate. Linear gradient elution was used and the detection wavelength was 260 nm. 3 impurities were found in tenofovir. The established method was accurate and reproducible, and it can be applied for the related substances test in tenofovir.
