Current progress in quantitative detection of microRNA
10.11665/j.issn.1000-5048.20150104
- VernacularTitle:microRNA定量检测方法的研究进展
- Author:
Shenrong ZHU
1
;
Xuri WU
;
Yijun CHEN
Author Information
1. 中国药科大学生命科学与技术学院化学生物学研究室
- Publication Type:Journal Article
- Keywords:
microRNA;
quantitative detection;
exponential amplification reaction;
rolling circle amplification;
enzyme-assisted microRNA detection
- From:
Journal of China Pharmaceutical University
2015;46(1):40-49
- CountryChina
- Language:Chinese
-
Abstract:
With the recent development of investigating biological functions of microRNA(miRNA), the importance of miRNA in the biological processes including developmental biology, metabolic regulation, disease progression and treatment has been well recognized. Due to its the instability, low level of expression and minor difference in sequence, more sophisticated analytical methods for rapid and easy quantitative detection of miRNA with strong specificity and high sensitivity play an important role in the study of the biological functions of miRNA. This review analyzes and compares recent quantitative methods to detect miRNA, with an attempt to provide a foundation for choosing an appropriate method to quantitatively analyze miRNA.
