Rhein:a novel matrix for profiling and imaging of endogenous metabolites by matrix-assisted laser desorption/ionization-mass spectrometry
10.11665/j.issn.1000-5048.20160616
- VernacularTitle:基于MALDI-MS技术的新基质大黄酸在代谢组学研究中的应用
- Author:
Hui YE
1
;
Yun WANG
;
Lin WANG
;
Xiaowei XU
;
Mengqiu WU
;
Guoxiu CAO
;
Yan LIANG
;
Guangji WANG
Author Information
1. 中国药科大学药物代谢动力学重点实验室
- Publication Type:Journal Article
- Keywords:
MALDI-MS;
mass spectrometry imaging;
novel matrix;
rhein;
metabonomics
- From:
Journal of China Pharmaceutical University
2016;47(6):727-733
- CountryChina
- Language:Chinese
-
Abstract:
MALDI-MS is an emerging technique that enables rapid profiling of endogenous substances in a high throughput. Nevertheless, its application to metabolite analysis is often hindered by the presence of massive matrix background peaks using conventional matrices. Herein rhein was introduced as a novel matrix for MALDI-MS analysis of cation metabolites. Several strong and weak basic metabolites were measured using rhein. Compared to the previously reported ionless matrix NSA and conventional matrix CHCA, clean MALDI-MS spectra were obtained devoid of matrix signals. Subsequently, we applied this matrix to profile a biologically complex sample, mice intestinal contents. In addition, we have validated the applicability of rhein to MALDI-MS imaging, which allows for simultaneous mapping of hundreds of metabolites from single mouse ileum section. In summary, the discovery of rhein as a novel MALDI matrix enables efficient and reliable profiling and imaging of endogenous metabolites from biological samples with no matrix interferences, which will greatly promote the utility of MALDI-MS-based platform for metabolome studies.
