Comparison of two new on-line methods on screening peroxynitrite scavengers in Radix Scrophulariae
- VernacularTitle:两种筛选玄参中清除过氧亚硝基活性成分的在线检测方法比较研究
- Author:
Ying WANG
1
;
Youhua CHEN
;
Jin Qi
;
boyang YU
Author Information
1. 中国药科大学 江苏省中药评价与转化重点实验室
- Collective Name:10.11665/j.issn.1000-5048.20160509
- Publication Type:Journal Article
- Keywords:
peroxynitrite;
chemiluminescence;
Radix Scrophulariae;
on-line detection
- From:
Journal of China Pharmaceutical University
2016;47(5):560-560
- CountryChina
- Language:Chinese
-
Abstract:
Two simple and efficient methods have been developed for screening and identification of natural peroxynitrite scavengers in Radix Scrophulariae (RS). Method I was based on HPLC-DAD-(luminol-peroxynitrite)-CL techniques combined with Q-TOF MS/MS analysis, while method II was based on the pre-column reaction with peroxynitrite followed by HPLC separation with Q-TOF MS/MS analysis. Five active constituents, P1(decaffeoylacteoside), P9(eoside), I6(6″-O-feruloylharpagide), P11(cis-acteoside)and P13(angoroside), were found to possess potential peroxynitrite-scavenging activity by method I, while P9 and P13 were also screened by method II. Method I requires more complex apparatus, but has advantages on simple detection and high sensitivity. Method II requires simpler apparatus than method I, but with more tedious detection and lower sensitivity. However, the methods established above would provide new ways for rapid detection of natural peroxynitrite-scavenging compounds in RX complex matrices.
