Improvement of NET and 5-HTT expression involved in the anti-depressive effect of valproate
10.11665/j.issn.1000-5048.20160414
- VernacularTitle:丙戊酸钠抗抑郁作用涉及改善海马NET和5-HTT表达
- Author:
Huizhi FEI
1
;
Xiaoya HU
;
Hongmei QIU
;
Dan LIU
;
Qixin ZHOU
Author Information
1. 重庆三峡医药高等专科学校药理学教研室
- Publication Type:Journal Article
- Keywords:
chronic mild unpredicted stress(CUMS);
antidepressive;
valproate;
norepinephrine transporter;
serotonin transporter
- From:
Journal of China Pharmaceutical University
2016;47(4):474-478
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed at investigating the correlations between antidepressive effect of valproate and improvements of NET and 5-HTT expression in depressive rats leaded by chronic mild unpredicted stress(CUMS)with solitary condition. Sixty male SD rats were divided into normal group(NG), model group(MG), valproate treated-normal group(VNG), and valproate treated-model group(VMG), randomly. The changes of depressive behaviors were evaluated by the open-field test and force swimming test. The contents of MDA, activities of SOD and CAT in serum, the mRNA and protein expression of NET, 5-HTT in hippocampus were determined by biochemical methods, Real-time PCR and Western Blot, respectively. Results showed that CUMS can significantly decrease the activities in open-field test, SOD and CAT activities in serum, expression of 5-HTT in hippocampus, and obviously increase the immobility time in force swimming test, the level of MDA and expression of NET. The treatment of valproate obviously improved the changes induced by CUMS. However, the treatment of valproate had no significant influences on behaviors of NG rats. So, it revealed that improvements of the mRNA and protein expression of NET, 5-HTT may be involved in the antidepressive effect of valproate.
