Metabolites of hypericin in rats in vivo

VernacularTitle:金丝桃素在大鼠体内的代谢产物
Author: Juanzhi ZHAO ^{1
,

2} ; Cong WANG ; Jindian LI ; Dongjian ZHANG ; Jian ZHANG ; Zhiqi YIN
Author Information

1. 中国药科大学天然药物化学教研室&
2. 天然药物活性组分与药效国家重点实验；江苏省中医药研究院转化医学实验室
Publication Type:Journal Article
Keywords: hypericin; metabolites; HPLC-TOF/MS; glycosylation; isolation and identification
From: Journal of China Pharmaceutical University 2016;47(4):457-462
CountryChina
Language:Chinese
Abstract: The study aimed to separate and identify the metabolites of hypericin in the bile and necrotic tissues in rats. After intravenous injection of 10 mg/kg hypericin, 0-12 h bile of normal rats and 24 h necrotic liver of rats with reperfused hepatic infarction were collected, and metabolites of rats were analyzed by high performance liquid chromatography coupled with electrospray tandemtime of flight mass spectrometry(HPLC-TOF/MS). The prototype(M0)and three glycosylation metabolites(M1, M2, M3)of hypericin in rat bile and the parent compound in rat necrotic liver were detected and identified. Results indicated that prototype and glycosylation of hypericin were the major metabolic form in rat bile and the parent compound was found only in necrotic tissues.