Trimethyl chitosan nanoparticles coated with Pluronic F-127 for oral insulin delivery system
10.11665/j.issn.1000-5048.20160409
- VernacularTitle:普朗尼克F-127包裹的三甲基壳聚糖纳米传递系统
- Author:
Yaxian ZHENG
1
;
Wunan ZHANG
;
Liping HE
;
Ruinan WU
;
Wei SHAN
;
Min LIU
;
Yuan HUANG
Author Information
1. 四川大学华西药学院，靶向药物与传递系统教育部重点实验室
- Publication Type:Journal Article
- Keywords:
Pluronic F-127;
N-trimethyl chitosan;
nanoparticles;
mucosal barrier
- From:
Journal of China Pharmaceutical University
2016;47(4):442-447
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this investigation was to develop Pluronic F-127 coated N-trimethyl chitosan nanoparticles(F-S NPs)of insulin as the model drug and asses their penetration of the mucosal barriers. Single factor screening was used to optimize the formulations of nanoparticles and the nanoparticles were characterized. Their particle size, Zeta potential, encapsulation efficiencies and drug loading were assayed to be(240. 6±6. 51)nm, (10. 42±1. 60)mV, (43. 39±2. 83)% and(3. 39±0. 57)%, respectively. The impact of PF-127 on mucin binding in vitro and nanoparticles′s transport in freshly obtained mucus were also evaluated. The mucin affinity of F-S NPs was significantly reduced when compared to that of the N-trimethyl chitosan nanoparticles(S NPs), i. e. , 28% of the latter. And F-S NPs was found to have an improved mucosal penetrating capability. Mucus-secreting HT29-MTX-E12(E12)cell monolayer was selected to investigate their cellular uptake. F-S NPs exhibited higher penetration coefficient than both free insulin and S NPs in mucus-secreting epithelium cells, i. e. , 16-fold and 1. 4-fold, respectively. Data suggest that F-S NPs be potential carriers to cross mucosal barriers and enhance the cellular uptake of insulin.
