Purification, identification and hypoglycemic activity in vitro of a novel peptide from red deer antler
10.11665/j.issn.1000-5048.20160320
- VernacularTitle:一种新型马鹿茸多肽纯化、鉴定及体外降血糖活性
- Author:
Ning JIANG
1
;
Shuangjian ZHANG
;
Jing ZHU
;
Jing SHANG
;
Xiangdong GAO
Author Information
1. 中国药科大学生命科学与技术学院；湖北民族学院生物科学与技术学院
- Publication Type:Journal Article
- Keywords:
red deer antler;
polypeptides;
insulin β cell HepG2 cell line;
hypoglycemic activity
- From:
Journal of China Pharmaceutical University
2016;47(3):363-367
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed at isolating a peptide with hypoglycemic activity from dried red deer antler. A peptide from dried red deer antler of Tarim in Xingjiang was extracted with acetate buffer(pH 3. 5), followed by precipitation with ethanol, ultrafiltration and separation via ion exchange chromatography, gel filtration and reversed phase chromatography. The molecular mass of the peptide was detected via MALDI TOF/MS and the peptide was identified by LC-MS/MS. The activities of the peptide to promote islet β cells proliferation and recovery the damage induced by streptozotocin(STZ)were detected by MTT, and the effect of the peptide on glucose consumption of hepatocyte was determined. The peptide, named as CAP, was obtained from dried red deer antler through purification with the molecular mass of 6 804 via MALDI TOF/MS. The result of LC-MS/MS and the comparison in relative database showed that CAP was a novel peptide. CAP stimulated the proliferation of pancreatic cells and recovery of STZ-damaged pancreatic cells, as well as increased glucose consumption of HepG2.
