Preparation and in vitro evaluation of albumin nanoparticles produced by thermal driven self-assembly
10.11665/j.issn.1000-5048.20160310
- VernacularTitle:热驱动自组装白蛋白纳米粒的制备及体外评价
- Author:
Fang LI
1
;
Liqun JIANG
;
Junbo XIN
;
Chunli ZHENG
;
Jiabi ZHU
;
Jianping LIU
Author Information
1. 中国药科大学药剂学教研室；盐城卫生职业技术学院药学院
- Publication Type:Journal Article
- Keywords:
thermal driven self-assembly;
albumin nanoparticles;
paclitaxel;
preparation;
formation mechanism;
intracellular degradation kinetics
- From:
Journal of China Pharmaceutical University
2016;47(3):303-310
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to prepare albumin nanoparticles by thermal driven self-assembly, and to investigate the formation mechanism, cellular uptake, the kinetics of cellular uptake and intracellular degradation, etc. By measuring the concentrations of thiol, amino and carboxyl groups, the formation mechanism of albumin nanoparticles was revealed. CCK-8 assay was performed to detect the cytotoxicity; inverted fluorescence microscope was used to observe the cellular uptake of the nanoparticles; while the fluorescence resonance energy transfer(FRET)method was applied to investigate the cellular uptake and intracellular degradation kinetics. The drug-loading capacity was investigated using paclitaxel(PTX)as the model drug. The results showed that the albumin nanoparticles produced by thermal driven self-assembly were safe, nontoxic, biodegradable and stabilized by intermolecular disulfide and amide bonds. The drug-loading study indicates that PTX can be highly encapsulated in the nanoparticles. Hence, thermal driven self-assembly method is green and easy to operate, and the albumin nanoparticles can be applied as a new delivery platform for anticancer drugs.
