Research on the necrosis target and imaging of necrotic myocardium of 131I-emodin and 131I-emodic acid
10.11665/j.issn.1000-5048.20160218
- VernacularTitle:131I-大黄素和131I-大黄素酸的坏死靶向性及成像坏死心肌的研究
- Author:
Qin WANG
1
;
Dongjian ZHANG
;
Jindian LI
;
Mengdan XU
;
Hong LIAO
;
Zhiqi YIN
;
Jian ZHANG
Author Information
1. 南京中医药大学药学院；江苏省中医药研究院转化医学实验室
- Publication Type:Journal Article
- Keywords:
131I-emodin 131I-emodic acid;
necrosis target;
myocardial infarction;
imaging
- From:
Journal of China Pharmaceutical University
2016;47(2):235-241
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study was to evaluate the necrosis target and imaging potential of necrotic myocardium of 131I-emodin and 131I-emodic acid. The iodogen coating method was used to radioiodinate emodin and emodic acid with iodine-131. Mice model of muscular necrosis and rat model of myocardial infarction(MI)were established to evaluate the necrosis affinity and imaging potential of 131I-emodin and 131I-emodic acid. Mice were sacrificed at 2, 12 and 24 h after injection respectively. The radioactive uptake in major organs and necrotic muscle were calculated by a γ-counter. At 6 h after administration, SPECT/CT imaging of necrotic myocardium in rats, biodistribution detection, histopathological analysis were applied to evaluate their necrosis affinity and imaging potential. The results of biodistribution from mice demonstrated that 131I-emodin and 131I-emodic acid showed peculiar necrosis target and exhibited an obvious clearance of radioactivity from normal organs. On SPECT/CT images, relatively high uptake as a hot spot was shown in the heart of the model rat, while no obvious uptake was observed in the heart of the control rat. The radioactivity ratios of necrotic to normal myocardium of 131I-emodin and 131I-emodic acid amounted up to 9. 72 and 13. 14 by quantitative autoradiography analysis, respectively. These results suggested that 131I-emodin and 131I-emodic acid possess the necrosis target and imaging potential of necrotic myocardium.
