Effect of block of AGEs-RAGE pathway on the migration of VSMCs
10.11665/j.issn.1000-5048.20160212
- VernacularTitle:AGEs-RAGE通路阻断对血管平滑肌细胞迁移能力的影响
- Author:
Min XIANG
1
;
Yaqin ZHANG
;
Pingping WU
;
Zhenyu GAO
Author Information
1. 苏州卫生职业技术学院药学教研室；苏州市检验医学生物技术重点实验室
- Publication Type:Journal Article
- Keywords:
advanced glycation endproducts;
receptor for advanced glycation endproducts;
diabetes mellitus;
VSMCs;
migration;
target;
signal transduction
- From:
Journal of China Pharmaceutical University
2016;47(2):199-203
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of block of AGEs-RAGE pathway on the migration of aortic vascular smooth muscle in diabetic rats and its possible mechanisms, vascular smooth muscle cells(VSMCs)cells were pre-stimulated by antibody of RAGE, and then stimulated by AGEs. Transwell assay was adopted to assay migration of VSMCs. Proliferation of VSMCs and expression of p27 were analyzed by MTT and ELISA, respectively. The change of ROS level in VSMCs was defermined by DCFH assay, the expression of NOX1 mRNA was determined by RT-PCR assay. Results indicated that the AGEs induction for migration of VSMCs was significantly inhibited after treatment by RAGE antibody(P< 0. 01), which blocked the AGEs-RAGE pathway, and the inhibition of migration was stronger than that of proliferation. The ROS level was decreased(P< 0. 01), and the expression of NOX1 mRNA was decreased, yet the expression of P27 protein was not changed greatly. Block of AGEs-RAGE pathway by antibody of RAGE can inhibit the migration of VSMCs, and the mechanism may be related with the decrease of NOX1 mRNA and then down to the level of intracellular oxidative stress in VSMCs.
