Chemical constituents from the leaves of Anisopus mannii and the melanogenesis inhibitory activities
10.11665/j.issn.1000-5048.20180606
- VernacularTitle:Anisopus mannii 叶的化学成分和抑制黑色素生成活性研究
- Author:
Feng YE
1
;
Qinglin LYU
;
Wanfang ZHU
;
Feng FENG
;
Jie ZHANG
Author Information
1. 中国药科大学中药学院
- Publication Type:Journal Article
- Keywords:
Anisopus mannii;
chemical constituents;
structural identification;
melanogenesis-inhibitory activity
- From:
Journal of China Pharmaceutical University
2018;49(6):676-681
- CountryChina
- Language:Chinese
-
Abstract:
The phytochemical studies on the leaves of Anisopus mannii led to the isolation of seven compounds by silica gel, ODS, DIAION HP-20, Sephadex LH-20 colunmn chromatographer, their structures were elucidated on the basis of the spectroscopic analyses(NMR, HRMS)and the comparisons with the literatures as 3β-acetoxylup-20(29)-ene(1), 1-acetoxy-2-isopropyl-1-tridecene(2), rutin(3), 3, 6′-diferuloylsucrose(4), 3-O-β-D-glucopyranosyl-3-β-hydroxyolean-12-en-28-oic acid 28-O-[α-L-rhamnopyra- nosyl-(1→2)-β-D-glucopyranosyl] ester(5), conduritol A(6), hoyacarnoside I(7). Meanwhile, the isolated compounds were evaluated for their inhibitory activities against melanogensis in B16 melanoma cells, as the results, all compounds exhibited melanogenesis-inhibitory activity and compound 5 showed a strongest activity(Melanin content: (27. 4±3. 5)%, Cell Viability: (54. 9±5. 6)% with a concentration of 30 μmol/L)which could be further developed.