Antiviral activities of flavonoids and diterpenoids isolated from Isodon eriocalyx(Dunn.)Hara
10.11665/j.issn.1000-5048.20180510
- VernacularTitle:毛萼香茶菜中黄酮和二萜化合物的分离制备及其抗病毒活性
- Author:
Jun WANG
1
;
Lingqiao CAI
;
Dong YAN
;
Min GUO
;
Qing WANG
;
Jian ZHANG
;
Zhiqi YIN
Author Information
1. 中国药科大学中药学院中药制药系；江苏省中医药研究院转化医学实验室
- Publication Type:Journal Article
- Keywords:
Isodon eriocalyx(Dunn.)Hara;
ent-kauranoid;
flavonoid;
antiviral activity
- From:
Journal of China Pharmaceutical University
2018;49(5):580-586
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to isolate two flavonoids and two diterpenoids from Isodon eriocalyx(Dunn. )Hara and investigate their antiviral activities in vitro. Firstly, four compounds were isolated from the ethanol extract of I. eriocalyx by silica gel, ODS and RP-HPLC chromatography, and then identified by spectral data as isothymusin(1), cirsimaritin(2), coetsoidin A(3), and maoecrystal D(4). Next, in vitro antiviral activities against influenza(H1N1/H3N2)and respiratory syncytial virus(RSV)was investigated for the ethanol extract of I. eriocalyx and four compounds. Results showed that the ethanol extract of I. eriocalyx and four compounds exhibited inhibitory effects against influenza H1N1. Especially, compounds 1 and 2 showed better activities with EC50 of(14. 45±4. 90)and(24. 54±3. 82)μmol/L. However, they showed weak inhibitory effects against influenza H3N2 and RSV. These results demonstrate that the ethanol extract of I. eriocalyx and compounds 1- 4 have antiviral activity, especially against influenza H1N1. The flavonoids may be one kind of the effective antiviral substances. This study provides a scientific basis for the clinical application of I. eriocalyx.
