Advances in the application of three-dimensional tumor spheroids model in the mechanism study of drug resistance
10.11665/j.issn.1000-5048.20180502
- VernacularTitle:三维肿瘤球模型应用于肿瘤耐药机制研究进展
- Author:
Leilei GUO
1
;
Yurui XU
;
Lei ZHANG
;
Yiwen DONG
;
Shuting LIN
;
Xinghai NING
;
Xiaoxuan LIU
Author Information
1. 中国药科大学天然药物活性组分与药效国家重点实验室；中国药科大学药物科学研究院高端药物制剂与材料研究中心
- Publication Type:Journal Article
- Keywords:
tumor spheroid;
drug resistance;
microenvironment
- From:
Journal of China Pharmaceutical University
2018;49(5):521-527
- CountryChina
- Language:Chinese
-
Abstract:
In comparison with the traditional two-dimensional tumor cell culture, the three-dimensional tumor spheroid culture can not only provide with an in vivo-like growth environment for tumor cells, but also maintain maximum cell activities. Therefore, the three-dimensional tumor spheroid culture is widely used in oncology research. In particular, the three-dimensional tumor cells retain the material and structural basis of the microenvironment of tumor in vivo, which is closer to the actual physiological environment, allowing it to be an ideal in vitro model for evaluating the tumor treatment response and drug resistance in tumors. This review summarizes the mechanisms of drug resistance in three-dimensional tumor cells, especially those induced by the morphology and microenvironment of three-dimensional tumor spheres, and puts forward the problems existing in the current three-dimensional tumor cells model, as well as the future development direction.
