Evaluating cerebral endothelial dysfunction induced by amyloid based on the time series model
10.11665/j.issn.1000-5048.20180411
- VernacularTitle:基于时间序列模型评估淀粉样蛋白引起的脑血管内皮功能损伤
- Author:
Chengyan ZHU
1
;
Haochen LIU
;
Hua HE
;
Xiaoquan LIU
Author Information
1. 中国药科大学药代动力学教研室
- Publication Type:Journal Article
- Keywords:
time series model;
cerebral endothelial dysfunction;
amyloid;
calcium ion channels
- From:
Journal of China Pharmaceutical University
2018;49(4):456-462
- CountryChina
- Language:Chinese
-
Abstract:
Time series model was developed to investigate the effect and contributions of related biomarkers on the cerebral endothelial dysfunction induced by beta amyloid(Aβ). HCMEC/D3 was incubated with 2. 5 μmol/L Aβ for 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24 h, and biomarkers including cytosolic calcium ion, mitochondrial membrane potential(MMP), endothelial nitric oxide synthase(eNOS)and cell viability were determined. Time series model was established to assess the dynamic relationship between the related biomarkers and cell viability and the contribution of different biomarkers to cell damage. Impulse response analysis indicated that after a positive impact on cytosolic calcium ion, cell viability decreased and this impact continued to decline; after a positive impact on endothelial nitric oxide synthase and mitochondrial membrane potential, cell viability increases, which increased rapidly in the early stage, and the rate decreased in later stage. The result of variance decomposition showed that the cytosolic calcium ion played a major role in cerebral endothelial dysfunction induced by Aβ. Combined with the model study, it is concluded that the intervention on the level of cytosolic calcium ion at the early stage may be the possible way to slow the disease progression.
