Detection of prostate specific antigen-based on dual signal amplified electrochemical immunoassay
10.11665/j.issn.1000-5048.20180408
- VernacularTitle:基于双重信号放大的电化学免疫法对前列腺特异性抗原的检测
- Author:
Xue LI
1
;
Yongling AI
;
Qunye HE
;
Gongjun YANG
;
Jing WANG
Author Information
1. 中国药科大学医药生物功能材料重点实验室
- Publication Type:Journal Article
- Keywords:
square wave stripping voltammetry;
GSH-CdTe quantum dots;
AuNPs;
reduced graphene oxide;
dual signal prostate specific antigen
- From:
Journal of China Pharmaceutical University
2018;49(4):433-440
- CountryChina
- Language:Chinese
-
Abstract:
A sandwiched electrochemical immunoassay based on the AuNPs@GSH-CdTe as a signal label, which formed by GSH-CdTe QDs and AuNPs, with dual signal amplified by reduced graphene oxide and AuNPs was proposed for the sensitive detection of prostate specific antigen(PSA). Through a sandwich immunoreaction, the target PSA and AuNPs@GSH-CdTe labeled Ab1 were captured to rGO/AuNPs-Ab2 surface. After the HNO3-dissolution step, square wave stripping voltammetry(SWSV)analysis of the captured CdTe QDs was used to quantify the concentration of PSA. In this system, AuNPs possessedlarge specific surface and good biocompatibility, which could effectively expand the amount of antigen and GSH-CdTe QDs loading and signals amplifying, while rGO played a synergistic amplification role due to its large specific surface. The proposed method showed good linearity ranging from 0. 5 to 200 ng/mL with the detection limits of 5. 0 pg/mL. It also showed excellent selectivity, good reproducibility, satisfactory stability. In addition, the method was successfully applied to the determination of real samples. The result was satisfactory and the recovery could fall in 98. 20%- 106. 2%, which represented a novel approach for versatile detection of tumor markers.
