Effect of triterpenic acid-enriched fraction from Cyclocarya paliurus on high glucose-induced pancreatic α cells insulin resistance
10.11665/j.issn.1000-5048.20180212
- VernacularTitle:青钱柳三萜酸对高糖所致的胰岛α细胞胰岛素抵抗的影响
- Author:
Yiting WANG
1
,
2
;
Mengge ZHAO
;
Xueping SHENG
;
Jian ZHANG
;
Cuihua JIANG
;
Zhiqi YIN
Author Information
1. 中国药科大学天然药物化学教研室&
2. 天然药物活性组分与药效国家重点实验室
- Publication Type:Journal Article
- Keywords:
Cyclocarya paliurus;
triterpenic acid-enriched(TAE);
αTC1-6 cells;
insulin resistance;
type 2 diabetes
- From:
Journal of China Pharmaceutical University
2018;49(2):215-221
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect and possible mechanisms of triterpenic acid-enriched fraction from Cyclocarya paliurus(TAE)on glucagon secretion in insulin-resistance pancreatic α cells, the model of insulin resistance in αTC1-6 cells was induced by long term exposure to high glucose. Experimental groups were divided as follow: control(5. 5 mmol/L glucose), model(25 mmol/L), TAE(1, 5, 10 μg/mL), and TAE(10 μg/mL)plus wortmannin(10 nmol/L)group. The supernatant and lysate of treated cells were collected to determine glucagon secretion by ELISA kit. The mRNA and protein abundance of IRS-1, PI3K and Akt were measured by qPCR and Western blot analysis. Results showed that TAE could not only significantly reduce glucagon secretion induce by high glucose in a dose-dependent manner, but also remarkably increased the mRNA and protein abundance of IRS-1, PI3K and Akt in αTC1-6 cells. However, these effects of TAE were reversed by PI3K inhibitor wortmannin. In conclusion, it suggested that TAE could improve the insulin resistance induced by high glucose in pancreatic α cells which may be related with the activation of IRS-1/PI3K/Akt signaling pathway.
