Investigation of herb-drug interaction between Liuwei Dihuang Pills and nifedipine based on rat liver microsomes and HPLC fingerprints
10.11665/j.issn.1000-5048.20180209
- VernacularTitle:基于肝微粒体和指纹图谱研究六味地黄丸与硝苯地平的药物相互作用
- Author:
Mei ZHENG
1
;
Fulei LIU
;
Jingwei XUE
;
Wenyuan LIU
Author Information
1. 药物质量与安全预警教育部重点实验室(中国药科大学)
- Publication Type:Journal Article
- Keywords:
Liuwei Dihuang Pill;
nifedipine;
rat liver microsome;
chromatographic fingerprints;
drug interaction;
pharmacodynamics
- From:
Journal of China Pharmaceutical University
2018;49(2):195-201
- CountryChina
- Language:Chinese
-
Abstract:
A new method based on rat liver microsomes and chromatographic fingerprint comparison was established to investigate the possible herb-drug interactions between Liuwei Dihuang Pills(LDP)and nifedipine(NIF). LDP and NIF were incubated simultaneously with rat liver microsomes at 37 °C for 60 min in a shaking water bath. The separation was achieved on a C18 column using 0. 1% formic acid solution and acetonitrile as mobile phase with a liner gradient program. The flow rate was set at 1. 0 mL/min. Detection was achieved by UV light at 240 nm. To evaluate the interactions between LDP and NIF, the similarity of the fingerprinting chromatograms before and after co-incubation was calculated by similarity evaluation system for chromatographic fingerprint of TCM. Furthermore, pharmacokinetic experiments in rat suggested that there was no significant difference in the pharmacokinetic parameters of the typical components in LDP.
