Understanding the Influence Factors in Viral Nucleic Acid Test of 2019 novel Coronavirus (2019-nCoV)
10.3760/cma.j.issn.1009-8158.2020.0002
- VernacularTitle: 正确认识新冠病毒核酸检测的影响因素
- Author:
Mo XI
1
;
Qin WEI
2
;
Fu QIHUA
1
;
Guan MING
3
Author Information
1. National Children’s Medical Center-Shanghai Children’s Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China
2. Shanghai Rundarongjia Biological Ltd., Shanghai 200127, China
3. Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai 200040, China
- Publication Type:Journal Article
- Keywords:
Novel coronavirus pneumonia;
RNA viruses;
Molecular diagnostic techniques;
Root cause analysis;
Nucleic acid detection
- From:
Chinese Journal of Laboratory Medicine
2020;43(0):E002-E002
- CountryChina
- Language:Chinese
-
Abstract:
At present, the prevention and control of new coronavirus has entered a critical period. However, the use of quantitative real-time PCR (qRT-PCR) assays for the detection of viral nucleic acid, as a crucial diagnostic approach, has been doubted in clinical practice. Herein, we have reviewed the current status of epidemic prevention and control, latest development of detection technologies, disease characteristics, clinical sampling and transport. We have also discussed the factors that may affect the performance of viral nucleic acid detection, and suggested some effective methods to improve the detection performance of the assays.
