Cytocompatibility and cell proliferation evaluation of calcium phosphate-based root canal sealers
- Author:
Letícia Boldrin MESTIERI
1
;
Ivana Maria ZACCARA
;
Lucas Siqueira PINHEIRO
;
Fernando Branco BARLETTA
;
Patrícia Maria Polli KOPPER
;
Fabiana Soares GRECCA
Author Information
- Publication Type:Original Article
- Keywords: Root canal; Cell migration; Fibroblast; Endodontics
- MeSH: 3T3 Cells; Animals; Calcium; Cell Movement; Cell Proliferation; Cell Survival; Dental Pulp Cavity; Endodontics; Fibroblasts; Mice; Pemetrexed; Wound Healing; Wounds and Injuries
- From:Restorative Dentistry & Endodontics 2020;45(1):2-
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVES: This study aimed to evaluate the cell viability and migration of Endosequence Bioceramic Root Canal Sealer (BC Sealer) compared to MTA Fillapex and AH Plus.MATERIALS AND METHODS: BC Sealer, MTA Fillapex, and AH Plus were placed in contact with culture medium to obtain sealers extracts in dilution 1:1, 1:2 and 1:4. 3T3 cells were plated and exposed to the extracts. Cell viability and migration were assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT) and Scratch assay, respectively. Data were analyzed by Kruskal-Wallis and Dunn's test (p < 0.05).RESULTS: The MTT assay revealed greater cytotoxicity for AH Plus and MTA Fillapex at 1:1 dilution when compared to control (p < 0.05). At 1:2 and 1:4 dilutions, all sealers were similar to control (p > 0.05) and MTA Fillapex was more cytotoxic than BC Sealer (p < 0.05). Scratch assay demonstrated the continuous closure of the wound according to time. At 30 hours, the control group presented closure of the wound (p < 0.05). At 36 hours, only BC Sealer presented the closure when compared to AH Plus and MTA Fillapex (p < 0.05). At 42 hours, AH Plus and MTA Fillapex showed a wound healing (p > 0.05).CONCLUSIONS: All tested sealers demonstrated cell viability highlighting BC Sealer, which showed increased cell migration capacity suggesting that this sealer may achieve better tissue repair when compared to other tested sealers.
