Novel Associations between Related Proteins and Cellular Effects of High-Density Lipoprotein
- Author:
Seungbum CHOI
1
;
Yae Eun PARK
;
Eun Jeong CHEON
;
Kyeong Yeon KIM
;
Miso KIM
;
Soo jin ANN
;
Hye Min NOH
;
Jaeho LEE
;
Chan Joo LEE
;
Seung Taek LEE
;
Cheolju LEE
;
Ji Eun LEE
;
Sang Hak LEE
Author Information
- Publication Type:Original Article
- Keywords: Atherosclerosis; Cardiovascular diseases; Proteomics; Lipoproteins; Immunity
- MeSH: Amyloid; Angiotensinogen; Atherosclerosis; Cardiovascular Diseases; Cholesterol; Complement System Proteins; Immunoglobulin mu-Chains; Lipoproteins; Nitric Oxide; Plasma; Proteomics; Serine Proteases; Thyroxine-Binding Globulin; Vascular Cell Adhesion Molecule-1; Vitronectin
- From:Korean Circulation Journal 2020;50(3):236-247
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND AND OBJECTIVES: Recent studies have examined the structure-function relationship of high-density lipoprotein (HDL). This study aimed to identify and rank HDL-associated proteins involved in several biological function of HDL.METHODS: HDLs isolated from 48 participants were analyzed. Cholesterol efflux capacity, effect of HDL on nitric oxide production, and vascular cell adhesion molecule-1 expression were assessed. The relative abundance of identified proteins in the highest vs. lowest quartile was expressed using the normalized spectral abundance factor ratio.RESULTS: After adjustment by multiple testing, six proteins, thyroxine-binding globulin, alpha-1B-glycoprotein, plasma serine protease inhibitor, vitronectin, angiotensinogen, and serum amyloid A-4, were more abundant (relative abundance ratio ≥2) in HDLs with the highest cholesterol efflux capacity. In contrast, three proteins, complement C4-A, alpha-2-macroglobulin, and immunoglobulin mu chain C region, were less abundant (relative abundance ratio <0.5). In terms of nitric oxide production and vascular cell adhesion molecule-1 expression, no proteins showed abundance ratios ≥2 or <0.5 after adjustment. Proteins correlated with the functional parameters of HDL belonged to diverse biological categories.CONCLUSIONS: In summary, this study ranked proteins showing higher or lower abundance in HDLs with high functional capacities and newly identified multiple proteins linked to cholesterol efflux capacity.
