Significance of PD-L1 expression in carbon-ion radiotherapy for uterine cervical adeno/adenosquamous carcinoma
- Author:
Moito IIJIMA
1
;
Noriyuki OKONOGI
;
Nakako Izumi NAKAJIMA
;
Yukie MOROKOSHI
;
Hiroaki KANDA
;
Taiju YAMADA
;
Yusuke KOBAYASHI
;
Kouji BANNO
;
Masaru WAKATSUKI
;
Shigeru YAMADA
;
Tadashi KAMADA
;
Daisuke AOKI
;
Sumitaka HASEGAWA
Author Information
- Publication Type:Original Article
- Keywords: B7-H1 Antigen; Disease-free Survival; Heavy Ion Radiotherapy; Treatment Outcome; Uterine Cervical Neoplasms
- MeSH: Antigens, CD274; Biopsy; Carcinoma, Squamous Cell; Disease-Free Survival; Flow Cytometry; Heavy Ion Radiotherapy; Humans; Radiotherapy; Treatment Outcome; Uterine Cervical Neoplasms
- From:Journal of Gynecologic Oncology 2020;31(2):19-
- CountryRepublic of Korea
- Language:English
- Abstract: OBJECTIVE: Programmed cell death-ligand 1 (PD-L1) is expressed in tumor cells and has been shown to predict clinical outcomes of several types of malignancies. The aim of this study was to investigate the effects of carbon-ion (C-ion) beam irradiation on PD-L1 expression in human uterine cervical adeno/adenosquamous carcinoma (UCAA) cells and clinical samples and to identify the prognostic factors for outcomes after C-ion radiotherapy (CIRT).METHODS: The effects of C-ion irradiation on PD-L1 expression in human UCAA and cervical squamous cell carcinoma cells were examined by flow cytometry. We examined PD-L1 expression in UCAA biopsy specimens from 33 patients before CIRT started (pre-CIRT) and after 12 Gy (relative biological effectiveness [RBE]) irradiation (post-12Gy-C) in 4 fractions of CIRT to investigate the correlation between PD-L1 status and clinical outcomes.RESULTS: The PD-L1 expression was upregulated by C-ion beam in a dose-dependent manner in HeLa and SiHa cells through phosphorylated Chk1. The overall frequencies of pre-CIRT and post-12Gy-C PD-L1 positivity were 45% (15/33) and 67% (22/33), respectively. The post-12Gy-C PD-L1 expression was significantly elevated compared to the pre-CIRT PD-L1 expression. There was no significant relationship between the pre-CIRT PD-L1 status and clinical outcomes, such as local control (LC), progression-free survival (PFS), and overall survival (OS). However, the post-12Gy-C PD-L1 expression had better correlation with PFS, but not with LC and OS.CONCLUSION: CIRT can induce PD-L1 expression in UCAA and we propose that PD-L1 expression after starting CIRT may become as a predictive prognostic marker in CIRT for UCAA.
