Oxidative Stress Modulates the Expression Pattern of Peroxiredoxin-6 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Bronchial Epithelial Cells
10.4168/aair.2020.12.3.523
- Author:
Hyun Jae SHIM
1
;
So Young PARK
;
Hyouk Soo KWON
;
Woo Jung SONG
;
Tae Bum KIM
;
Keun Ai MOON
;
Jun Pyo CHOI
;
Sin Jeong KIM
;
You Sook CHO
Author Information
1. Division of Allergy and Clinical Immunology, Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. yscho@amc.seoul.kr
- Publication Type:Original Article
- Keywords:
Asthma;
airway;
inflammation;
peroxiredoxin-6;
oxidative stress;
hydrogen peroxide;
post-translational modifications;
proteasome inhibitor
- MeSH:
Asthma;
Cycloheximide;
Epithelial Cells;
Homeostasis;
Humans;
Hydrogen;
Hydrogen Peroxide;
Immunoprecipitation;
Inflammation;
Lung;
Lysine;
Oxidative Stress;
Proteasome Inhibitors;
Protein Processing, Post-Translational;
Serine
- From:Allergy, Asthma & Immunology Research
2020;12(3):523-536
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Reduction-oxidation reaction homeostasis is vital for regulating inflammatory conditions and its dysregulation may affect the pathogenesis of chronic airway inflammatory diseases such as asthma. Peroxiredoxin-6, an important intracellular anti-oxidant molecule, is reported to be highly expressed in the airways and lungs. The aim of this study was to analyze the expression pattern of peroxiredoxin-6 in the peripheral blood mononuclear cells (PBMCs) of asthmatic patients and in bronchial epithelial cells (BECs).METHODS: The expression levels and modifications of peroxiredoxin-6 were evaluated in PBMCs from 22 asthmatic patients. Phosphorylated and acetylated peroxiredoxin-6 in hydrogen peroxide-treated human BECs was detected using immunoprecipitation analysis. The expression level of peroxiredoxin-6 was also investigated in BECs treated with hydrogen peroxide. Cycloheximide and proteasome inhibitors were used to determine whether peroxiredoxin-6 is degraded by proteasomes.RESULTS: Peroxiredoxin-6 expression was significantly reduced in the PBMCs of asthmatic patients compared to control subjects. Distinct modification patterns for peroxiredoxin-6 were observed in the PBMCs of asthmatic patients using 2-dimensional-electrophoresis. The levels of phosphorylated serine and acetylated lysine in peroxiredoxin-6 were significantly increased in the BECs following hydrogen peroxide treatment. The level of peroxiredoxin-6 expression was reduced in hydrogen peroxide-stimulated BECs, presumably due to proteasomes.CONCLUSIONS: The expression of peroxiredoxin-6, which is down-regulated in the immune cells of asthmatic patients and BECs, can be modified by oxidative stress. This phenomenon may have an effect on asthmatic airway inflammation.
