Virus Isolation from the First Patient with SARS-CoV-2 in Korea
- Author:
Wan Beom PARK
1
;
Nak Jung KWON
;
Su Jin CHOI
;
Chang Kyung KANG
;
Pyoeng Gyun CHOE
;
Jin Yong KIM
;
Jiyoung YUN
;
Gir Won LEE
;
Moon Woo SEONG
;
Nam Joong KIM
;
Jeong Sun SEO
;
Myoung don OH
Author Information
- Publication Type:Brief Communication
- Keywords: 2019 Novel Coronavirus; Culture; Microscopy, Electron; Phylogeny; Korea
- MeSH: China; Coronavirus; Crowns; Genome; Humans; Korea; Microscopy, Electron; Microscopy, Electron, Transmission; Phylogeny; Vero Cells
- From:Journal of Korean Medical Science 2020;35(7):84-
- CountryRepublic of Korea
- Language:English
- Abstract: Novel coronavirus (SARS-CoV-2) is found to cause a large outbreak started from Wuhan since December 2019 in China and SARS-CoV-2 infections have been reported with epidemiological linkage to China in 25 countries until now. We isolated SARS-CoV-2 from the oropharyngeal sample obtained from the patient with the first laboratory-confirmed SARS-CoV-2 infection in Korea. Cytopathic effects of SARS-CoV-2 in the Vero cell cultures were confluent 3 days after the first blind passage of the sample. Coronavirus was confirmed with spherical particle having a fringe reminiscent of crown on transmission electron microscopy. Phylogenetic analyses of whole genome sequences showed that it clustered with other SARS-CoV-2 reported from Wuhan.
