Angiopoietin-like protein 3 induces podocytes actin rearrangement via integrin beta 3
10.3760/cma.j.issn.1001-7097.2017.01.007
- VernacularTitle: 血管生成素样蛋白3通过整合素β3信号通路参与足细胞骨架重排
- Author:
Xia GAO
1
;
Hong XU
;
Jia RAO
;
Haimei LIU
;
Junchao LIU
Author Information
1. Department of Nephrology, Children's Hospital of Fudan University, Shanghai 201102, China
- Publication Type:Journal Article
- Keywords:
Integrin beta3;
Cytoskeleton;
Podocytes;
Angiopoietin-like protein 3
- From:
Chinese Journal of Nephrology
2017;33(1):43-47
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether Angiopoietin-like protein 3 (ANGPTL3) is involved in podocyte actin rearrangement, and to analyze whether integrin β3 signal pathway is a key in ANGPTL3 inducing actin rearrangement.
Methods:The cultured podocytes were divided into six groups: wild type, ADR treated, ADR+Dex, MOCK, ANGPTL3-cDNA, miRNA, and AD+miRNA group. (1) We observed actin cytoskeleton using Invitrogen reagents with confocal microscopy; (2) Actin cytoskeleton after blocking β3 on podocytes was; (3) The expression of total FAK and p-FAK was through Western blotting.
Results:(1) The wild type podocyte's cytoskeleton is arranged orderly. After ADR treatment, podocyte's actin are rearranged and weaken (P<0.05). There was no significant difference in actin arrangement between knock-down and MOCK group. In ANGPTL3-cDNA group the podocyte actin was also significantly rearranged; on the contrary, in miRNA+ADR group, the actin rearrangement never obviously happened (P<0.05). (2) Over-expression of ANGPTL3 podocytes blocked integrin β3 did not happen actin rearrangement. (3) The expression of p-FAK significantly increased in over-expression ANGPTL3 podocytes.
Conclusion:ANGPTL3 is a key in inducing actin rearrangement. Intergrin β3 maybe a central pathway in ANGPTL3's role with podocytes.
