Study of compound glutathione inosine injection effect on expression of regucalcin in liver tissue of rats with immune hepatic fibrosis
10.3760/cma.j.issn.1007-3418.2019.08.008
- VernacularTitle: 谷胱甘肽复方注射液对免疫性肝纤维化大鼠肝组织钙调素表达的影响
- Author:
Junying LIU
1
;
Yang LIU
;
Han LI
;
Chaohui SHI
;
Zulan LIANG
;
Linghui WANG
;
Yu ZHANG
;
Yuan ZHAO
;
Yumei FAN
;
Bin WU
;
Yanzhang YU
Author Information
1. Zhoukou Central Hospital, Zhoukou 466000, China
- Publication Type:Journal Article
- Keywords:
Rats;
Immune hepatic fibrosis;
Regucalcin;
Compound glutathione inosine injection
- From:
Chinese Journal of Hepatology
2019;27(8):628-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the change in expression of anti-senescence marker protein calmodulin (RGN) in liver tissues of rats with immune hepatic fibrosis, and to observe the effect of compound glutathione inosine injection (CGII) on it.
Methods:Rat liver fibrosis model was induced by intraperitoneal injection of porcine serum, and CGII intervention was administered at the appropriate time. Rat liver tissues were stained with HE and Masson. RGN and protein expression at mRNA in liver tissues was detected by fluorescence quantitative PCR and immunohistochemistry. One-way Anova was used for measurement data. LDS test was used for two-way comparison, and pathological semi-quantitative results were analyzed by rank-sum test.
Results:The relative expression of RGN mRNA and protein in liver tissue of fibrotic rats was 82.23 ± 15.21 and 12.52 ± 3.23, respectively, which were significantly lower than that of normal rats 176.39 ± 11.35 and 59.23 ± 9.13 (P < 0.01). The degree of liver fibrosis in fibrotic rats after CGII intervention was significantly lower than fibrotic rats. The relative expression of RGN mRNA and protein in the intervention group was 168.78 ± 21.31 and 46.42 ± 4.71, respectively, which were significantly higher than fibrosis and spontaneous recovery group. The difference was statistically significant (P < 0.01). The relative expression of RGN mRNA and protein in the spontaneous recovery group was 86.23 ± 17.16 and 14.34 ± 5.16, which was higher than model group. The difference was not statistically significant (P > 0.05).
Conclusion:The expression of RGN in liver tissue of rats with hepatic fibrosis induced by porcine serum is decreased, while the expression of RGN increases with the decrease of fibrosis after CGII intervention, suggesting that the protein may play an important role in the development of liver fibrosis.
