Minocycline Inhibits Caspase-Dependent Cell Death Pathway and is Neuroprotective against Hippocampal Damage after Kainic Acid-Induced Seizure in Mice.
- Author:
Ha Young SHIN
1
;
Yang Je CHO
;
Kyoung Joo CHO
;
Hyun Woo KIM
;
Hyun Jung KIM
;
Gyung Whan KIM
;
Byung In LEE
;
Kyoung HEO
Author Information
1. Department of Neurology, Yonsei University College of Medicine, Brain Research Institute, Seoul, Korea. Kheo@yumc.yonsei.ac.kr
- Publication Type:Original Article
- Keywords:
Minocycline;
Seizure;
Apoptosis;
Caspase;
Cytochrome c
- MeSH:
Adult;
Animals;
Apoptosis;
Blotting, Western;
Caspase 3;
Cell Death*;
Cytochromes c;
Cytosol;
DNA Fragmentation;
Epilepsy;
Humans;
In Situ Nick-End Labeling;
Kainic Acid;
Male;
Mice*;
Mice, Inbred ICR;
Minocycline*;
Models, Animal;
Neurons;
Seizures*;
Viola
- From:Journal of Korean Epilepsy Society
2006;10(1):3-10
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Despite current acceptance of its neuroprotective property, whether the minocycline affords neuroprotection or how it protects neurons against seizures in the animal model of epilepsy is not clear. This prompts us to investigate whether minocycline is neuroprotective against kainic acid (KA)-induced seizure in mice through inhibition of caspase-dependent mitochondrial apoptotic pathways. METHODS: Adult male ICR mice were subjected to seizures by intrahippocampal KA injection with treatment of vehicle or minocycline. For cell death analysis, histological analysis using cresyl-violet staining, TdT-mediated dUTP-biotin nick end labeling (TUNEL), and histone-associated DNA fragmentation analysis were performed. Evaluation of cytochrome c, cleaved caspase-3, and caspase-3 activity were also performed. RESULTS: Hippocampal neuronal death was evident by cresyl violet staining, TUNEL, and cell death assay in vehicle-treated mice after KA injection; however, there was significant reduction of cell death in the minocycline-treated group. Significant decrease of both cytosolic translocation of cytochrome c and subsequent activation of caspase-3 after treatment of minocycline were demonstrated by Western blot analysis, immunohistochemical staining, and caspase-3 activity assay. CONCLUSION: This study suggests that minocycline may be neuroprotective against hippocampal damage after KA-induced seizure through inhibition of caspase-dependent cell death pathways.
