Experimental study of toluidine blue-dextran-40 as lymphatic tracer in head and neck region
10.3760/cma.j.issn.1002-0098.2019.07.009
- VernacularTitle: 甲苯胺蓝-低分子右旋糖酐作为头颈部淋巴走行示踪剂的实验研究
- Author:
Fengtong LIU
1
;
Wei SHANG
2
;
Wei LI
2
;
Shengbiao WAN
3
;
Jun CUI
1
Author Information
1. Department of Oral and Maxillofacial Surgery, Jinan Stomatological Hospital, Jinan 250001, China
2. Department of Oral and Maxillofacial Surgery, the Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China
3. Department of Pharmaceutical Chemistry, School of Medicine, China Ocean University, Qingdao 266000, China
- Publication Type:Journal Article
- Keywords:
Coloring agents;
Lymph nodes;
Staining and labeling;
Head and neck neoplasms;
Biopsy
- From:
Chinese Journal of Stomatology
2019;54(7):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility and application value of toluidine bule-dextran-40 (TB-Dex-40) as the tracer for lymphatic system in head and neck region.
Methods:Twenty healthy adult New Zealand white rabbits were equally divided into two groups: the experimental group (TB-Dex-40 group, n=10) and the control group (TB group, n=10). Rabbits in experimental group received submucosal injection of 1.0% (0.14 mOsm/L) TB-Dex-40, and the control group received injection of 1.0% (32.60 mOsm/L) TB.The staining time and fading time of lymphatic vessels and lymphnodes in the neck region were recorded, and the diffusion ranges of the two dyes in the tongue region were measured. Lymph nodespecimen were collected for pathological examination after 10 min, 1 hour and 4 weeks of staining. The experimental animals were sacrificed before and 4 weeks after the experiment. After death, organs of heart, lung, liver and kidney were examined pathologically.
Results:TB-Dex-40 reached sentinel lymph node (SLN) and stained lymphatic vessels at an average of (21.67±0.19) s after injection, while in control group was(3.22±0.34) s (P<0.01). The time difference between the two dyes reaching sentinel lymph nodes was statistically significant.The durations from lymphatic staining to marked fading were (19.70±1.34) min in experimental group and (14.30±0.95) min in control group, respectively.The difference was statistically significant (P<0.01). SLN staining by TB-Dex-40 was still evident after 4 weeks, while TB staining has completely faded after 2 d.The average ranges of diffusionin tongue were (10.50±1.08) mm in experimental group and (20.00±1.05) mm in controlgroup, respectively. The difference was statistically significant (P<0.01).No abnormalities were found in blood test and pathological examination of main organs.
Conclusions:TB-Dex-40 has high specificity forstaining lymphatic vessels and is a good tracer with potential clinical value.
