Effect of TCF21 on proliferation, migration and vascular production of breast cancer cells
10.3760/cma.j.issn.0529-5807.2019.04.009
- VernacularTitle: 转录因子21对乳腺癌细胞增殖、迁移、血管生成的影响
- Author:
Piwu LI
1
;
Haiyan WANG
1
;
Lingling XU
2
Author Information
1. Department of Pharmacy, Jinan Third People′s Hospital, Jinan 250132, China
2. Department of Intensive Care, Jinan Central Hospital, Jinan 250013, China
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Transcription factors;
Cell proliferation;
MDA-MB-231
- From:
Chinese Journal of Pathology
2019;48(4):307-311
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of transcription factor 21 (TCF21) on the proliferation, apoptosis, invasion and migration and angiogenesis of human breast cancer cells (MDA-MB-231).
Methods:TCF21 overexpressing plasmid was transfected into human breast cancer cell line MDA-MB-231 by liposome transfection, and Western blot was used to detect whether the transfection was successful. The effects of TCF21 overexpression on proliferation, apoptosis, invasion and migration and angiogenesis of MDA-MB-231 were detected by MTT, DAPI, Transwell and CAM.
Results:The expression of TCF21 protein in Western blot showed that the TCF21 overexpression plasmid was successfully transfected into MDA-MB-231 cells. Overexpression of TCF21 inhibited the proliferation of tumor cells and inhibit the proliferation of 48 h cells after transfection. Overexpression of TCF21 inhibited tumor invasion and migration and angiogenesis, and promoted apoptosis of tumor cells. The results of Western blot showed that the protein expression of MMP-9, VEGFA and p-VEGFR2 decreased significantly after overexpression of TCF21.
Conclusions:TCF21 can inhibit the proliferation, invasion and migration, angiogenesis and apoptosis of MDA-MB-231 cells. It is suggested that TCF21 can be used as a potential site for clinical diagnosis and treatment of breast cancer.
