A study on alterations in mitochondrial biological characteristics during cellular senescence of human embryonic lung fibroblasts
10.3760/cma.j.issn.0253-9624.2019.03.014
- VernacularTitle: 人胚肺成纤维细胞衰老过程中线粒体生物学性状变化的研究
- Author:
Jianji GAO
1
;
Caiyun LAI
1
;
Wenjuan ZHANG
1
;
Xingfen YANG
2
Author Information
1. Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China
2. Food Safety and Health Research Center/School of Public Health, Southern Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
Mitochondria;
Cell aging;
Human embryonic lung fibroblasts
- From:
Chinese Journal of Preventive Medicine
2019;53(3):309-315
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the alterations of mitochondrial biological characteristics during both cellular replicative and premature senescence induced by hydrogen peroxide in human embryonic lung fibroblasts (HEFs).
Methods:The premature senescence was induced by 400 μmol/L H2O2 once a day at the same time and with 2 hours each time, after four consecutive days the premature senescence models were classified into premature senescence initiation group (PSi) and premature senescence persistence group (PSp). Based on the life span of HEFs, the cell replicative senescence was divided into five groups included young-age (22 PDL), middle-age (35 PDL), replicative senescence (49 PDL), PSi and PSp. The mitochondrial distribution, relative content, adenosine triphosphate (ATP) contents, 8-hydroxydeoxyguanosine (8-OHdG) levels, the relative mitochondrial transcription factor A (TFAM) as well as mitochondrial DNA methyltransferase 1 (mtDNMT1) mRNA levels, mtDNA copy number, the relative TFAM protein level and the total enzyme activity of mitochondrial DNA methyltransferases (mtDNMTs) were detected in five senescence groups.
Results:The mtDNA copy number, 8-OHdG contents, level of mtDNMT1 mRNA and mtDNMTs activity in 49 PDL group were higher than those in 22 PDL group (all P values <0.05); The level of 8-OHdG in PSi was higher than that in 22 PDL group (P<0.05); The ATP contents, mtDNA copy number, the mRNA and protein expression levels of TFAM and mtDNMTs activity of PSp were higher than those in 22 PDL group (all P values<0.05).
Conclusion:During the cellular senescence of HEFs, the higher mtDNA copy number and mtDNMTs activity were common features regardless of replicative or premature senescence, with possibility that oxidative stress was involved in modifying the occurrence of premature senescence.
