Influence of TNF-α on the immunomodulatory property of laryngeal mucosa mesenchymal stromal cells′
10.3760/cma.j.issn.1673-0860.2019.03.008
- VernacularTitle: 肿瘤坏死因子α对喉黏膜间充质干细胞免疫调节能力的影响
- Author:
Huan LIU
1
;
Shiyu LIU
2
;
Xinyu QIU
2
;
Zhihong DENG
1
;
Yan JIN
2
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University, Xi′an 710032, China
2. Research and Development Center for Tissue Engineering, the Fourth Military Medical University, Xi′an 710032, China
- Publication Type:Journal Article
- Keywords:
Laryngeal mucosa;
Mesenchymal stromal cells;
Tumor necrosis factor-alpha;
Immunomodulation
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2019;54(3):203-208
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of tumor necrosis factor-alpha(TNF-α)on the immunoregulatory capacity of laryngeal mucosal mesenchymal stromal cells (LM-MSCs) and its potential molecular mechanism, and provide a theoretical basis for the study of chronic laryngitis.
Methods:LM-MSCs were separated from epiglottal mucosa. The LM-MSCs cells were directly co-cultured with T cells in vitro to detect the immunomodulatory property of LM-MSCs. After long-term stimulation with inflammatory factors TNF-α in vitro, the differences were compared in the immunomodulatory ability of LM-MSCs between normal LM-MSCs and TNF-α stimulated LM-MSCs. The expression of general control non-repressed protein5(GCN5), FAS, FASL in normal LM-MSCs and TNF-α stimulated LM-MSCs was detected by Western blot and quantitative real-time RT-PCR(RT-qPCR).
Results:After chronic stimulation of TNF-α, the RNA relative expression of GCN5 was 0.31±0.03 (3 days) and 0.53±0.06 (7 days) compared with control group, showing significant difference (F=13.45, P<0.05). The percentage of LM-MSC-induced T cell apoptosis was 6.27%±0.81% (3 days) and 4.99%±0.52% (7 days) in chronic stimulation group compared with control group 10.02%±1.02%. There is a significant difference among these groups (F=11.13, P<0.05). Moreover, the ability of LM-MSCs to induce T cell apoptosis is regulated by GCN5.
Conclusion:With the chronic stimulation of TNF-α, the expression of GCN5 in LM-MSCs is decreased, thus impairing its immunoregulatory capacity.
