Genetic characteristic of hemagglutinin of avian influenza A (H7N9) virus in Guizhou Province in 2017
10.3760/cma.j.issn.0253-9624.2019.02.020
- VernacularTitle: 2017年贵州省H7N9禽流感病毒血凝素基因特性分析
- Author:
Yonghu WAN
1
;
Li ZHUANG
1
;
Lijuan REN
1
;
Qunni ZHENG
1
;
Lin FU
1
;
Zhuzhou SHAN
2
;
Fengfeng PEI
2
;
Weijia JIANG
1
;
Guangpeng TANG
1
;
Shijun LI
1
Author Information
1. Clinical Laboratory of Institute for Communicable Disease Control and Prevention, Guizhou Center for Disease Control and Prevention, Guiyang 550004, China
2. Clinical Laboratory of Institute for Communicable Disease Control and Prevention, Qiandongnan Center for Disease Control and Prevention, Kaili 556000, China
- Publication Type:Journal Article
- Keywords:
Influenza in birds;
Hemagglutinin glycoproteins, influenza virus;
Genes;
Avian influenza A (H7N9) virus
- From:
Chinese Journal of Preventive Medicine
2019;53(2):229-232
- CountryChina
- Language:Chinese
-
Abstract:
The number of H7N9 bird flu cases was high and the situation was grim in guizhou province in 2017. To understand the molecular characteristics of the hemagglutinin gene (HA) and the risk of human infection with avian influenza virus A(H7N9) in Guizhou Province, 2017. Homology, genetic evolution and pivotal sites related to receptor binding regions, pathogenicity and potential glycosylation of 14 avian influenza viruses A(H7N9) were analyzed by a series of bioinformation softwares. It was cleared that there was 95.9%-100% similarity among 14 strains in nucleotide of the HA gene, and there were 96.8%-97.8% and 96.8%-97.9% similarities with vaccine strains A/Shanghai/2/2013 and A/Anhui/1/2013 recommended by WHO, respectively. Phylogenetic analysis showed that 14 HA genes were directly evolved in the Yangtze River Delta evolution branch, but they could be derived from five diffenrent strains. Then 13 of 14 strains cleavage site sequences of HA protein revealed they were low pathogenic avian influenza viruses, while A/Guizhou-Weining/CSY01/2017 was high pathogenic avian influenza virus. Mutation G186V at the receptor binding sites in the HA was found in all 14 strains, and mutation Q226L in 13 strains besides A/Guizhou-Weining/CSY01/2017. All five potential glycosylation motifs in the HA were conservative.
