Promoting migration and apoptosis of glioma cells by macrophage migration inhibitory factor
10.3760/cma.j.issn.0529-5807.2019.02.006
- VernacularTitle: 巨噬细胞移动抑制因子影响胶质瘤细胞迁移、凋亡的机制
- Author:
Xiaozhen JIANG
1
;
Kui DENG
2
;
Meiling HUA
1
;
Zhi LI
3
;
Shan XU
1
Author Information
1. Department of Pathology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
2. Department of Orthopedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
3. Department of Pathology, Guangdong General Hospital, Guangzhou 510080, China
- Publication Type:Journal Article
- Keywords:
Glioma;
Macrophage migration-inhibitory factors;
Cell movement;
Apoptosis
- From:
Chinese Journal of Pathology
2019;48(2):108-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of macrophage migration inhibitory factor (MIF) on the biology of glioma U87MG and U251 cells.
Methods:Silencing MIF gene expression in U87MG cells by RNA interference was monitored by Western blot. MIF low expressing U251 cells were treated at different concentrations of recombinant human MIF (rhMIF) and scratching test and flow cytometry were used to detect cell migration and apoptosis. The protein expression of bcl-2, bax, AKT, p-AKT was detected by Western blot.
Results:The ability of migration and anti-apoptosis of U87MG cells silenced by siRNA decreased significantly, and the expression levels of p-AKT and anti-apoptotic protein bcl-2 also decreased; in contrast, the expression level of apoptosis protein bax increased. With increase of rhMIF treatment concentration, the expression levels of MIF protein, p-AKT and bcl-2 in U251 cells were gradually enhanced, whereas the level of apoptosis protein bax was inhibited.
Conclusion:MIF promotes cell migration and inhibits apoptosis of both U87MG and U251 cells, likely through the regulation of PI3K/AKT signaling pathway.
