Experimental study of silybin-phospholipid complex intervention on amiodarone-induced fatty liver in mice
10.3760/cma.j.issn.1007-3418.2019.01.010
- VernacularTitle: 水飞蓟宾-磷脂复合物干预胺碘酮诱导的小鼠脂肪肝的实验研究
- Author:
Shuangshuang SUN
1
,
2
;
Yinxia WU
3
;
Mingliang CHENG
4
;
Chengwei CHEN
1
;
Yanshen PENG
5
;
Qi MIAO
5
;
Zhaolian BIAN
5
;
Xiaojin WANG
1
;
Qingchun FU
1
Author Information
1. The 405th Hospital of PLA, Liver Disease Research Center, Shanghai 200235, China
2. Department of Infectious Diseases, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
3. Lishui Central Hospital, Zhejiang 323000, China
4. Department of Infectious Diseases, The Affiliated Hospital of Guizhou Medical University, Gui yang 550004, China
5. Department of Gastroenterology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China
- Publication Type:Journal Article
- Keywords:
Amiodarone;
Fatty liver;
Drug-induced liver injury;
Models, animal;
Silybin-phospholipid complex
- From:
Chinese Journal of Hepatology
2019;27(1):45-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To probe into the mechanism and interventional effects of silybin-phospholipid complex on amiodarone-induced steatosis in mice.
Methods:Eight-week-old male C57BL/6 mice were divided into three groups (5 mice in each group): a control group (WT) with normal diet, a model group with amiodarone 150mg/kg/d by oral gavage (AM), and an intervention group on amiodarone 150mg/kg/d combined with silybin-phospholipid complex(AM+SILIPHOS. All mice were fed their assigned diet for one week. Then, one week later, serum alanine aminotransferase, aspartate aminotransferase, triglyceride, total cholesterol and high-density lipoprotein were detected of each group. A liver pathological change was observed by oil red O and H&E staining. Ultrastructural pathological changes of hepatocytes were observed to evaluate the intervention effect by transmission electron microscopy. RT-q PCR was used to detect the expression of peroxisome proliferator-activated receptor alpha and its regulated lipid metabolism genes CPTI, CPTII, Acot1, Acot2, ACOX, Cyp4a10 and Cyp4a14 in liver tissues. Intra-group comparison was done by paired t-test. One-way ANOVA was used for comparison between groups and semi-quantitative data were tested using Mann-Whitney U test.
Results:Oil Red O and H&E staining results of liver tissue in the intervention group showed that intrahepatic steatosis was significantly reduced when compared to model group. Transmission electron microscopy showed that the model group had pyknotic nuclei, mitochondrial swelling, structural damage, and lysosomal degradation whereas the intervention group had hepatic nucleus without pyknosis, reduced mitochondrial swelling and slight structural damage than that of model group. RT-q PCR results showed that the expression of peroxisome proliferator-activated receptor alpha, CPTI, CPTII, Acot1, Acot2, ACOX, Cyp4a10 and Cyp4a14 were increased in the model group but the expression of CPTI, Cyp4a14, Acot1 and peroxisome proliferator-activated receptor alpha were decreased in the intervention group (P < 0.05).
Conclusion:Silybin-phospholipid complex can alleviate amiodarone-induced steatosis, and its mechanism may play a role in protecting mitochondrial function and regulating fatty acid metabolism. Thus, silybin-phospholipid complex has potential intervention effect on amiodarone-induced fatty liver.
