Effects of CD34+ selected stem cells for the treatment of poor graft function after allogeneic stem cell transplantation
10.3760/cma.j.issn.0253-2727.2018.10.008
- VernacularTitle: 纯化供者CD34+细胞输注治疗单倍型造血干细胞移植后移植物功能不良12例临床分析
- Author:
Xinhong FEI
1
;
Junbao HE
;
Haoyu CHENG
;
Yuming YIN
;
Weijie ZHANG
;
Shuqin ZHANG
;
Xiaocan WANG
;
Jingbo WANG
Author Information
1. Aerospace Center Hospital of Peking University, Beijing 100049, China
- Publication Type:Journal Article
- Keywords:
CD34+-selected stem cell;
Poor graft function;
Allogeneic stem cell transplants;
Graft-versus-host disease
- From:
Chinese Journal of Hematology
2018;39(10):828-832
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the efficacy and safety of purified CD34+ stem cell boost in the treatment of poor graft function (PGF) after allogeneic hematopoietic stem cell transplantation (HSCT) .
Methods:12 patients with poor graft function, reported in our hospital during January 2014 to March 2018, were retrospectively analyzed; The donors of 12 patients were HLA mismatched family members, and all treated with donor purified CD34+ stem cell after G-CSF mobilization, calculating and statistical analyzing the purity of separation and the recovery rate of CD34+ stem cells. The related complications and the recovery of blood cells after infusion were observed.
Results:The purity of CD34+ cells in the separation products was 92.0% (44.0%-97.0%) , and the recovery rate was 55.0% (45.0%-96.7%) . The median number of CD34+ cells was 1.9 (0.9-4.4) ×106/kg with CD3+ cells as 0.6 (0.3-2.0) ×104/kg. The median durations of white blood cells, platelet and red blood cells recoveries were 18 (14-39) , 29 (16-153) and 60 (9-124) days, respectively. All 12 patients didn’t experience serious adverse reactions in the process of infusion, 10 patients achieved hematopoietic recovery, 1 case partial remission, 1 case no recovery, without occurrence of aggravated infection, graft versus host disease and other complications.
Conclusion:The infusion of donor purified CD34+ stem cell was a safe and effective method for PGF after allogeneic HSCT.
