Circular RNA circHIPK3 acts as the sponge of microRNA-124 to promote human oral squamous cell carcinoma cells proliferation
10.3760/cma.j.issn.1002-0098.2018.08.009
- VernacularTitle: 环状RNA circHIPK3调控微RNA124表达促进口腔鳞状细胞癌细胞增殖的研究
- Author:
Jun WANG
1
;
Siyu ZHAO
2
;
Shaobo OUYANG
2
;
Zikun HUANG
3
;
Qing LUO
3
;
Lan LIAO
2
Author Information
1. Department of Oral Prosthodontics, Affiliated Stomatological Hospital of Nanchang University & The Key Laboratory of Oral Biomedicine, Jiangxi Province, Nanchang 330006, China (Present address: Department of Oral and Maxillofacial Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China)
2. Department of Oral Prosthodontics, Affiliated Stomatological Hospital of Nanchang University & The Key Laboratory of Oral Biomedicine, Jiangxi Province, Nanchang 330006, China
3. Department of Clinical Laboratory, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China
- Publication Type:Journal Article
- Keywords:
Oral sprays;
Circular RNA;
MicroRNAs
- From:
Chinese Journal of Stomatology
2018;53(8):546-551
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression and clinical significance of circular RNA circHIPK3 in oral squamous cell carcinoma (OSCC), analyze the effect of circHIPK3 on the proliferation of OSCC cells.
Methods:The expression of circHIPK3 in OSCC tissues, adjacent non-cancerous tissues and OSCC cell lines were detected by quantitative real-time polymerase chain reaction (qPCR). The correlations between the expression of circHIPK3 in OSCC tissues and the clinicopathological features were analyzed as well. circHIPK3-specific siRNA si-circHIPK3 and negative control siRNA si-NC were designed and synthesised and used to transfect CAL27 and SCC15 cells respectively. The proliferation capacity of CAL27 and SCC15 cells after transfection with si-circHIPK3 was detected by CCK-8 assay. The expression of miR-124 in OSCC was detected by qPCR, and the correlation between expression of circHIPK3 and the expression of miR-124 was analyzed. Using qPCR to detect the expression of miR-124 in CAL27 and SCC15 cells after transfection with si-circHIPK3 and si-NC respectively. Furthermore, using CCK-8 assay to detect the proliferation capacity of CAL27 and SCC15 cells after transfection with si-NC, si-circHIPK3, miR-124 mimic, si-circHIPK3+miR-124 inhibitor.
Results:The expression of circHIPK3 in OSCC tissues [2.23 (1.86, 3.00)] was significantly higher than that of the adjacent non-cancerous tissues [1.05 (0.85, 1.26)] (U=1 094, P=0.000). The expression of circHIPK3 in CAL27 (3.02±0.51) and SCC15 cells (3.16±0.75) was higher than those of human normal oral keratinocytes (hNOK) (1.26±0.30) (P=0.000). The expression of circHIPK3 was found to be closely associated with TNM stage (P<0.05) and tumor grades (P<0.05). Knockdown of circHIPK3 can inhibit proliferation of CAL27 and SCC15 cells (P<0.05). The expression of miR-124 in OSCC tissues (0.61±0.35) was significantly lower than that in adjacent non-cancerous tissues (1.13+0.39) (t=-5.36, P<0.05). Correlation analysis showed that the expression of circHIPK3 in OSCC was negatively correlated with the expression of miR-124 (r=-0.767, P<0.001). Moreover, down-regulation of miR-124 rescued the phenotype induced by knockdown of circHIPK3.
Conclusions:The expression of circHIPK3 in OSCC was increased, and silencing of circHIPK3 expression can inhibit the proliferation of OSCC cells. Our results suggest that circHIPK3 may play a key role in the occurrence and development process of OSCC through the regulation of miR-124 expression.
