MicroPET-CT study of the association between odor stimulation and olfactory related brain activation in rats
10.3760/cma.j.issn.1673-0860.2018.07.005
- VernacularTitle: 气味刺激与大鼠嗅觉相关脑区激活的microPET－CT研究
- Author:
Xing GAO
1
;
Zhifu SUN
1
;
Xiaoguang YAN
1
;
Baihan SU
1
;
Linyin YAO
1
;
Jia LIU
1
;
Yichen GUO
1
;
Qianwen LYU
1
;
Xiaoli ZHANG
2
;
Yongxiang WEI
1
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
2. Department of Nuclear Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China
- Publication Type:Journal Article
- Keywords:
Rats;
Olfactory cortex;
Fluorodeoxyglucose F18;
PET-CT
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(7):507-511
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Using 18F-fluorodeoxyglucose (18F-FDG) and microPET-CT to test the feasibility of 18F-FDG PET-CT for validation of olfactory function of rats with standard phenethyl alcohol (PEA) and isovaleric acid (IVA) odors stimulation. To verify the possibility of 18F-FDG PET-CT as a new objective examination method for olfactory function.
Methods:Six healthy Sprague-Dawley (SD) male rats were selected with a weight of 250-300 g. First of all, buried food pellet test (BFT) was used to confirm the normal olfactory function of rats. Then in the next 3 days, after the intravenous injection of 18F-FDG (18 MBq/100 g), awaken rats were placed in a ventilated plexiglas cage for 30 min. Subsequently, pure air (the first day), PEA (the second day) and IVA (the third day) were delivered. After odor stimulation for 30 min, rats were performed by a static PET-CT under anesthesia. Images reconstructed were assessed by SPM method and analyzed by VBM method. Data was analysied by paired t test.
Results:Activation regions of rat′s brain after PEA stimulation included bed nucleus and insula. Activation regions of rat′s brain after IVA stimulation included olfactory bulb, anterior olfactory nucleus, amygdala, entorhinal cortex, olfactory cortex, piriform cortex, insula, prefrontal cortex, cingulate cortex and bed nucleus (P<0.005, Ke>20 voxels).
Conclusions:Through microPET-CT, we can observe that olfactory stimulation with different odors can induce metabolic activation in different regions of rat′s brain, which was in concordance with olfactory regions. The olfactory related brain regions of rats have strong responses to odor stimulation of IVA.
