Research progress of Lgr5-positive stem cells in the formation of organoid in 3D culture
10.3760/cma.j.issn.1673-0860.2018.06.020
- VernacularTitle: Lgr5阳性干细胞在3D培养中形成类器官的研究进展
- Author:
Qingqing HE
1
;
Ao LI
1
;
Maohua WANG
2
;
Xia GAO
1
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Research Institute of Otolaryngology, 210008 Nanjing, China
2. Department of Otorhinolaryngology Head and Neck Surgery, Xiangya No.2 Hospital of Central South University, 410011 Changsha, China
- Publication Type:Review
- Keywords:
Stem cells;
Organoids;
Organculture techniques;
Regeneration
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2018;53(6):469-473
- CountryChina
- Language:Chinese
-
Abstract:
Stem cell is critical to regeneration of tissue or organ of human. How to promote repair or regeneration in the tissues/organ using its pluripotency is always an important issue. Lgr5-possitive cell is one type of the stem cell-like cells capable of pluripotent differentiation in various tissues/organs of both humans and mice. Current study showed that single or small amount Lgr5-possitive stem cells can grow and form a plurality of organs in 3D culture system, and some organs can present similar biological and physiological properties with the progenitor they were derived. These studies provided new insight into future orientation, for example, Lgr5-possitive inner ear cells were confirmed as inner ear pluripotent cells population, the experiences obtained from organoid studies of Lgr5-possitive cells have certainly showed potential in the future study of inner ear stem cells. This review will focus on the recent progress associated with Lgr 5-positive stem cells forming organoids in the 3D culture.
