The expression of KLK7 in pancreatic cancer and the effects on the biological behavior of pancreatic cancer cells
10.3760/cma.j.issn.0529-5815.2018.05.013
- VernacularTitle: KLK7在胰腺癌中的表达及其对胰腺癌细胞生物学行为的影响
- Author:
Suli ZHENG
1
;
Mengyu FENG
;
Gang YANG
;
Guangbing XIONG
;
Lianfang ZHENG
;
Taiping ZHANG
;
Yupei ZHAO
Author Information
1. Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Academy of Chinese Medical Sciences, Beijing 100730, China
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Animal experimentation;
Kallikrein 7;
Vascular invasion;
Chemosensitivity
- From:
Chinese Journal of Surgery
2018;56(5):391-397
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of KLK7 in pancreatic cancer and its clinical significance.
Methods:Immunohistochemistry was used to detect the expression of KLK7 protein in pancreatic cancer tissue microarray with 92 samples. Statistical analysis of the relationship between KLK7 and clinicopathological characteristics was finished. Pancreatic cancer cell lines were infected with lentiviuses in order to get cells with KLK7 stable overexpression.KLK7-siRNA was transfected into pancreatic cancer cells to knock down KLK7.Cell proliferation and chemosensitivity were detected by CCK-8 assay; Cell invasion and migration abilities were detected by Transwell assay. At the same time, subcutaneous xenograft tumor models were established in nude mice to observe the effect of KLK7 on tumor growth in nude mice. Data were statistically analyzed by rank sum test, χ2 test and Logistic regression analysis.
Results:The expression level of KLK7 in pancreatic cancer tissues was higher than that in paired adjacent tissues (P<0.05). KLK7 expression was correlated with vascular invasion(χ2=7.535, P<0.05). Further univariate and multivariate analysis showed that KLK7 expression was an independent risk factor for vascular invasion of pancreatic cancer(χ2=7.535, P<0.05). The overexpression of KLK7 in pancreatic cancer cell lines BxPC-3 and CFPAC can increase their proliferation abilities, reduce the chemosensitivity and promote their migration and invasion behaviour; The results of in vivo experiments showed that the volume of subcutaneously transplanted tumors in the overexpressing KLK7 group was significantly larger than that in the control group (t=4.479, P<0.05). The group of overexpressing KLK7 showed greater tumor weight than the control group(t=2.831, P<0.05).
Conclusions:The expression level of KLK7 in pancreatic ductal adenocarcinoma was higher than that in paired adjacent tissues and it is an independent risk factor for vascular invasion of pancreatic cancer.KLK7 can promote the proliferation of pancreatic cancer cells, reduce the chemosensitivity and increase the invasion and migration of pancreatic cancer cells.
