Expression and potential clinical significance of cytidine triphosphate: phosphocholine cytidylyltransferase-α in oral squamous cell carcinoma
10.3760/cma.j.issn.1002-0098.2018.04.008
- VernacularTitle: 磷酸胆碱胞苷酰转移酶α在口腔鳞状细胞癌中的表达及潜在临床意义
- Author:
Fachao LU
1
;
Xiaoyan XIE
1
;
Xiaomin YIN
2
;
Yijun GAO
1
Author Information
1. Dental Center, Second Xiangya Hospital, Central South University, Changsha 410011, China
2. Department of Stomatology, Xiangya Hospital, Central South University, Changsha 410008, China
- Publication Type:Journal Article
- Keywords:
Oral sprays;
Mouth neoplasms;
Cytidine triphosphate: phosphocholine cytidylyltransferase-α
- From:
Chinese Journal of Stomatology
2018;53(4):254-258
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examine the expression and potential clinical significance of CCT (cytidine triphosphate: phosphocholine cytidylyltransferase)-α in oral squamous cell carcinoma (OSCC).
Methods:Fifty-eight OSCC and paired adjacent non-malignant epithelia samples (between May 2016 and July 2016) were obtained from dental center, Second Xiangya Hospital, Central South University. CCT-α expression was examined by immunohistochemistry. The relationship between CCT-α and clinicopathological features of OSCC patients was analyzed. Quantitative real-time PCR and Western blot were performed to measure the expression of CCT-α mRNA and protein level in several OSCC cell line and two normal oral epithelial cell line.
Results:Immunohistochemistry showed that CCT-α positive staining was found in cell nuclear of OSCC cells and adjacent epithelial cells. CCT-α was positively expressed in OSCC, which was significantly higher than that adjacent to carcinoma tissues (P=0.000). The expression of CCT-α in oral squamous cell carcinoma was correlated with smoking, alcohol consumption, tumor size, differentiation degree and lymph node metastasis. The expression level of CCT-α protein was significantly increased in patients with a history of smoking and alcohol consumption (P=0.001, P=0.004). With the increase of tumor diameter, the expression of CCT-α protein was significantly increased (P=0.005). According to histopathological grade, the lower the degree of tumor differentiation, the higher the expression level of CCT-α protein (P=0.000). The expression of CCT-α protein was significantly higher in patients with lymph node metastasis compared with no lymph node metastasis (P=0.000). Quantitative real-time PCR results showed the CCT-α mRNA expression level was significantly higher in OSCC cells than that in normal oral epithelial cells (P=0.016). The protein expression level of CCT-α was significantly higher in OSCC cells than that in normal oral epithelial cells.
Conclusions:CCT-α may play a critical role in the carcinogenesis and development of OSCC.
