Protective Effects of Gabapentin on Allodynia and alpha2delta1-Subunit of Voltage-dependent Calcium Channel in Spinal Nerve-Ligated Rats.
10.3346/jkms.2009.24.1.146
- Author:
Tae Soo HAHM
1
;
Hyun Joo AHN
;
Chang Dae BAE
;
Han Seop KIM
;
Seung Woon LIM
;
Hyun Sung CHO
;
Sangmin M LEE
;
Woo Seog SIM
;
Jie Ae KIM
;
Mi Sook GWAK
;
Soo Joo CHOI
Author Information
1. Department of Anesthesiology and Pain Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. hyunjooahn@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Alpha 2 Delta-1 Subunit;
Gabapentin;
Neuropathic Pain;
Protective;
Calcium Channels
- MeSH:
Amines/administration & dosage/*therapeutic use;
Analgesics/administration & dosage/*therapeutic use;
Animals;
Calcium Channels/genetics/*metabolism;
Cyclohexanecarboxylic Acids/administration & dosage/*therapeutic use;
Disease Models, Animal;
Injections, Intraperitoneal;
Ligation;
Male;
Neuralgia/*drug therapy/metabolism;
Pain Measurement;
Protein Subunits/genetics/metabolism;
Rats;
Rats, Sprague-Dawley;
Spinal Nerves/surgery;
Up-Regulation;
gamma-Aminobutyric Acid/administration & dosage/*therapeutic use
- From:Journal of Korean Medical Science
2009;24(1):146-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was designed to determine whether early gabapentin treatment has a protective analgesic effect on neuropathic pain and compared its effect to the late treatment in a rat neuropathic model, and as the potential mechanism of protective action, the alpha2delta1-subunit of the voltage-dependent calcium channel (alpha2delta1-subunit) was evaluated in both sides of the L5 dorsal root ganglia (DRG). Neuropathic pain was induced in male Sprague-Dawley rats by a surgical ligation of left L5 nerve. For the early treatment group, rats were injected with gabapentin (100 mg/kg) intraperitoneally 15 min prior to surgery and then every 24 hr during postoperative day (POD) 1-4. For the late treatment group, the same dose of gabapentin was injected every 24 hr during POD 8-12. For the control group, L5 nerve was ligated but no gabapentin was administered. In the early treatment group, the development of allodynia was delayed up to POD 10, whereas allodynia was developed on POD 2 in the control and the late treatment group (p<0.05). The alpha2delta1-subunit was up-regulated in all groups, however, there was no difference in the level of the alpha2delta1-subunit among the three groups. These results suggest that early treatment with gabapentin offers some protection against neuropathic pain but it is unlikely that this action is mediated through modulation of the alpha2delta1-subunit in DRG.
