The expression of lncRNA UCA1 and its role on the proliferation and invasion in hepatic cancer and HepG2 cells
- VernacularTitle:lncRNAUCA1在肝癌中的表达及对肝癌HepG2 细胞增殖和侵袭的作用及机制
- Author:
Hai-bo, WANG
1
;
Zhong-lian LI
Author Information
1. Department of Hepatobiliary and Pancreatic Surgery II, Tianjin Nankai Hospital, Tianjin 300100, China
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
cell proliferation;
neoplasm invasiveness;
neoplasm metastasis;
lncRNA;
urothelial carcinoma-associated1(UCA1)
- From:
Tianjin Medical Journal
2018;46(7):682-686
- CountryChina
- Language:Chinese
-
Abstract:
Objective Toinvestigatetheeffectsoflongnon-codingRNAurothelialcarcinoma-associated1(lncRNA UCA1) silencing on cell proliferation and invasion of human hepatic cancer HepG2 cells, and its mechanism thereof. Methods TheexpressionoflncRNAUCA1wasanalyzedbyreal-timePCRin20tumortissueand20adjacentnon-tumor tissuesamplesofhepaticcancer.HepG2cellswasculturedin vitro,andlncRNAUCA1specificshorthairpinRNA(shRNA1 andshRNA2)wastransfected.CCK-8assay,TranswellassayandWound-healingassaywereusedtodetecttheeffectof lncRNAUCA1silencingoncellproliferation,invasionandmigrationofHepG2cells.TheeffectoflncRNAUCA1silencing onproteinandmRNAexpressionofCyclinD1,vascularendothelialgrowthfactor(VEGF),matrixmetalloproteinase(MMP)9,focaladhesionkinase(FAK)andIntegrinβ3onlncRNAUCA1silencingwasmeasuredbyreal-timePCRandWestern blotassay.Results TheexpressionofLncRNAUCA1washigherinhepaticcancertissues.ThesilencingoflncRNAUCA1 significantly inhibited the growth of HepG2 cells, and the abilities of cell invasion and migration were downregulated. Westernblotassayandreal-timePCRshowedthatexpressionsofCyclinD1,VEGF,MMP-9,FAKandIntegrinβ3were significantlyinhibited.Conclusion TheresultssuggestthattheabnormalexpressionoflncRNAUCA1isassociatedwith thedevelopmentofhumanhepaticcancer.ThesilencingoflncRNAUCA1couldsuppressthecellproliferation,invasionand migrationofhepaticcarcinomacells.
