Expression and correlation of Fra-1 and HMGA1 in laryngeal squamous cell carcinoma
10.3760/cma.j.issn.1673-0860.2017.12.010
- VernacularTitle: Fra－1与HMGA1基因在喉鳞状细胞癌中的表达及相关性研究
- Author:
Yunlong ZHANG
1
;
Xiaofei SONG
2
;
Yunjing DUAN
3
;
Ruili ZHAO
4
Author Information
1. The third Department of Intensive Care Unit, the First Hospital of Shijiazhuang, Shijiazhuang 050011, China
2. Department of Otorhinolaryngology, Hebei General Hospital, 050011, China
3. Department of Otorhinolaryngology, the First Hospital of Shijiazhuang, Shijiazhuang 050011, China
4. Department of Otorhinolaryngology, the Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China
- Publication Type:Journal Article
- Keywords:
Laryn;
Carcinoma, squamous cell;
Immunohistochemical;
Reverse transcription polymerase chain reaction;
Gene
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2017;52(12):927-932
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expressions of Fra-1 and HMGA1 in laryngeal squamous cell carcinoma and their correlation.
Methods:Immunohistochemistry and reverse transcription-polymer chain reaction (RT-PCR) were used to detect the expressions of HMGA1 and Fra-1 in laryngeal squamous carcinoma tissues in 47 cases and para-carcinoma tissues in 21 cases(the First Hospital of Shijiazhuang). The relationship between the gene expressions in carcinoma tissues and clinopathological parameters such as pathological grade, clinical stage, lymph metastasis, age and anatomic site and the relevance of the two gene expressions were analyzed. SPSS 13.0 software was used to analyze the data.
Results:The positive expression rates of Fra-1 and HMGA1 proteins in laryngeal squamous cancer tissue were 48.9% and 53.2%, which were respectively higher than the rates of 19.0% for Fra-1 (χ2=5.416, P<0.05) and of 23.8% for HMGA1 (χ2=5.083, P<0.05) in adjacent tissues. The expression of Fra-1 gene was correlation with pathological grade, clinical stage and lymph metastasis (t values were -1.079, -1.066 and -1.067, all P<0.05), but not with age and anatomic site (t values were -1.068 and -1.054, both P>0.05). The expression of HMGA1 gene was correlation with pathological grade, clinical stage, lymph metastasis and age (t values were -1.112, -1.065, -1.009 and -1.066, all P<0.05), but not with anatomic site (t=-1.036, P>0.05). The expressions of Fra-1 and HMGA1 gene were positively correlation (r=0.672, P<0.05).
Conclusions:In laryngeal squamous cancer, Fra-1 and HMGA1 are excessive expression, with a positive correlation between the expressions of both genes.
