Screening of adult Ph-like acute lymphoblastic leukemia by multiplex real-time quantitative PCR
10.3760/cma.j.issn.0253-2727.2017.11.011
- VernacularTitle: 多重实时荧光定量PCR法筛查成人Ph样急性淋巴细胞白血病
- Author:
Meizhen XU
1
;
Qiuyun FANG
;
Xiaoyuan GONG
;
Juan FENG
;
Yujiao JIA
;
Qinghua LI
;
Kaiqi LIU
;
Xingli ZHAO
;
Kun RU
;
Zheng TIAN
;
Kejing TANG
;
Min WANG
;
Jianxiang WANG
;
Yingchang MI
Author Information
1. Institute of Hematology&Blood Diseases Hospital, CAMS & PUMC, Tianjin 300020, China
- Publication Type:Journal Article
- Keywords:
Leukemia, B-cell, Ph-like;
Gene, CRLF2;
Multiplex RT-PCR
- From:
Chinese Journal of Hematology
2017;38(11):956-961
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the feasibility of multiplex real-time RT-PCR with fluorescent probes in early screening of Ph-like acute lymphoblastic leukemia (ALL) and analyze the clinical feature and prognos.
Method:A total of 118 adult B-ALL patients diagnosed between October 2010 and March 2016 were enrolled in this study. Multiplex RT-PCR was used to detect the Ph-like ALL related fusion gene and CRLF2 expression in 58 BCR-ABL and MLL rearrangement negative patients. The clinical features, treatment response and prognosis were analyzed in Ph-like fusion gene positive and/or CRLF2 over-expression patients.
Result:Among 58 patients, 9 patients (9/58, 15.5%) showed Ph-like ALL related fusion genes positive and 10 patients (10/58, 17.2%) showed CRLF2 over-expression. There were statistical differences in age, WBC count, immunophenotypes, cytogenetics and risk stratification among Ph-like fusion gene positive or CRLF2 over-expression patients, Ph+ patients, MLL+ patients and B-other patients. The 2-year overall survival rates were 65%, 47%, 64% and 74% respectively among these four groups (P=0.043) . The 2-year relapse free survival rates were 51%, 39%, 62% and 70% respectively among these four groups (P=0.010) .
Conclusion:Routine screening of Ph-like ALL by multiplex RTPCR is feasible.
