Drug resistance and dissemination of New Delhi metallo-β-lactamase 1 positive bacteria in a patient
10.3760/cma.j.issn.0253-9624.2017.10.005
- VernacularTitle: 携带新型金属β-内酰胺酶基因菌株在某患者体内播散及耐药研究
- Author:
Xiaofei ZHAO
1
,
2
;
Min YUAN
;
Xia CHEN
;
Xinfeng LIU
;
Deshan YU
;
Juan LI
Author Information
1. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention
2. State Key Laboratory of Infectious Disease Prevention and Control, Beijing 102206, China
- Publication Type:Journal Article
- Keywords:
Drug resistance;
Beta-lactamases;
Infection control
- From:
Chinese Journal of Preventive Medicine
2017;51(10):890-895
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The aim of this work was to report the surveillance and dissemination of NDM-1 positive bacteria in a patient and ward environment.
Methods:In 2010, during the therapy for a 51 years old patient, clinical and environmental samples were collected for carbapenem resistant bacterial culture, according to the clinical microbiological examination. Strains identification and antibiotic susceptibility were tested by VITEK Compact 2 system and E-test. The blaNDM-1 was detected by PCR and analyzed by sequencing. Plasmids containing blaNDM-1 were submitted to PFGE-S1 and Southern hybridization.
Results:During hospitalization from October 1st to November 4th, nine strains were isolated from blood, sputum, urine, fecal, and ward ground samples. The Klebsiella oxytoca, Raoultella planticola, and Acinetobacter baumannii were isolated from blood sample. The Klebsiella pneumonia and Acinetobacter baumannii were isolated from sputum sample. An Acinetobacter lwoffii was isolated from urine sample. An Escherichia coli was isolated from fecal sample. And the Acinetobacter lwoffii and Acinetobacter spp. were isolated from ward ground. Four strains were NDM-1 positive, which were Raoultella planticola (RpNDM1) isolated from blood, Escherichia coli (EcNDM1) isolated from fecal, Acinetobacter lwoffii (AlDNM1) and Acinetobacter spp. (AsNDM1) isolated from ward ground. Four NDM-1 positive strains were resistant to Piperacillin, Piperacillin tazobactam, Cefepime, Ceftriaxone, Ceftazidime, Imipenem, Meropenem, and Ertapenem. Southern hybridization revealed that blaNDM-1 were all located on plasmids in the four positive strains.
Conclusion:blaNDM-1 can transfer rapidly among different species, resulting in difficult to control and prevent. While isolating patient who is carrying NDM-1 positive strains, more attention should be paid to the disposal of patient's excreta, especially stool, should be paid more attention.
