Enhancement of thermal damage to EpCAM-positive tumor cells by novel aptamer-guided magnetic nanoparticles
10.3760/cma.j.issn.0253-3766.2017.10.002
- VernacularTitle: 新型核酸适配体介导的铁磁纳米颗粒增强上皮细胞黏附分子阳性肿瘤细胞的热疗效应
- Author:
Liyu ZHANG
1
;
Meng WANG
2
;
Haibin WU
3
;
Ying YANG
1
;
Qiao LI
4
;
Hongli SUN
1
Author Information
1. Shanxi Institute of Pediatric Diseases, Xi′an Children′s Hospital, Xi′an 710002, China
2. Department of Orthopaedics, 11 Military Hospital of China, Yining 835000, China
3. Science and Education Division, Xi′an Children′s Hospital, Xi′an 710002, China
4. Clinical Laboratory, Xi′an Children′s Hospital, Xi′an 710002, China
- Publication Type:Journal Article
- Keywords:
Neoplasms;
Aptamers;
Epithelial cell adhesion molecule;
Magnetic nanoparticles;
Thermal therapy
- From:
Chinese Journal of Oncology
2017;39(10):726-731
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the thermal damage to epithelial cell adhesion molecule(EpCAM)-positive tumor cells by novel aptamer-guided magnetic nanoparticles(AptNPs).
Methods:EpCAM aptamer SYL3C was connected to NPs via biotin-streptavidin reaction. The diameter of AptNPs were characterized by Dynamic Light Scattering(DLS). The binding feature of the aptamer to EpCAM-positive tumor cells was evaluated by Prussian blue dyeing. Thermal damage under alternative magnetic field was measured bylactate dehydrogenase (LDH). The apoptosis of EpCAM-positive tumor cells was detected by acridine orange/ethidium bromide (AO/EB) double staining.
Results:The average size of AptNPs was 282 nm. Flow cytometry and Prussian blue dyeing showed that AptNPs exhibited strong binding to the EpCAM-positive tumor cells but not to the EpCAM-negative tumor cells. Moreover, when incubated with 1.5×108 AptNPs under alternative electromagnetic fieldfor 5 hours, the viability of EpCAM-positive HCT116 cells and A549 cells was 28.9% and 54.4%, respectively, significantly lower than 76.7% of EpCAM-negative HepG2 cells (P<0.05).
Conclusions:AptNPs can improve the thermal damage to EpCAM-positive tumor cells, and may have potential utility in the development of tumor targeted therapy.
