Loss of BRCA associated protein 1 expression in malignant mesothelioma and its diagnostic application
10.3760/cma.j.issn.0529-5807.2017.10.008
- VernacularTitle: BRCA相关蛋白1在恶性间皮瘤中的表达缺失及其在鉴别诊断中的应用价值
- Author:
Zhenying GUO
1
;
Wenyong SUN
;
Carbone MICHELE
;
Gu ZHANG
;
Jinlin HU
;
Dan SU
;
Haining YANG
;
Weimin MAO
Author Information
1. Department of Pathology, Zhejiang Cancer Hospital, Hangzhou 310022, China
- Publication Type:Journal Article
- Keywords:
Mesothelioma;
BRCA1 protein;
Lung neoplasms;
Immunohistochemistry
- From:
Chinese Journal of Pathology
2017;46(10):699-703
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of BRCA-associated protein 1 (BAP1) in malignant mesothelioma, non-small cell lung cancer and carcinosarcoma, and its application in the differential diagnosis.
Methods:Twenty-two cases of malignant mesothelioma including 17 epithelioid type, 2 sarcomatoid type and 3 biphasic type were collected.As the study control, 80 non-small cell lung cancers infringement pleural membrane(including 40 lung adenocarcinomas and 40 lung squamous cell carcinomas) and 15 carcinosarcomas were included. BAP1 expression was detected using immunohistochemical method. A differential diagnosis antibody panel, including calretinin, WT1, CK5/6, D2-40, CAM5.2, CEA, TTF1, Napsin A, p63 and p40 was tested in all cases.
Results:All 80 cases of non-small cell lung cancer and 15 cases of carcinosarcoma were BAP1 positive. In contrast, 64% (14/22) of malignant mesotheliomas lost BAP1 expression (P<0.01). Addition of BAP1 to the mesothelioma marker panel, the diagnostic accuracy of malignant mesothelioma was enhanced to 93%. Focal expression of BAP1 in tumors suggested multiclonal evolution of mesothelioma.
Conclusions:Loss of BAP1 expression helps to confirm the diagnosis of malignant mesothelioma whereas all non-small cell lung cancer expresses BAP1. It is therefore recommended that BAP1 can be used in conjunction with other immunohistochemical markers to improve the diagnostic accuracy of malignant mesothelioma.
