Simultaneous determination of trichloroethylene and trichloroethanol in blood by liquid-liquid extraction-gas chromatography
10.3760/cma.j.issn.1001-9391.2017.10.020
- VernacularTitle: 血中三氯乙烯和三氯乙醇同时测定的液液萃取-气相色谱法
- Author:
Haipeng YE
1
;
Ji SHAO
;
Siwei TAN
;
Xiaoyue SHAN
;
Yanpeng SHI
Author Information
1. Hangzhou Occupational Disease Prevention and Control Hospital, Hangzhou 310014, China
- Publication Type:Journal Article
- Keywords:
Trichloroethylene;
Trichloroethanol;
Blood;
Liquid-liquid extraction;
Chromatography,gas
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2017;35(10):780-782
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for determing the trichloroethylene(TCE)and trichloroethanol(TCOH)in blood samples by liquid-liquid extraction-gas chromatography with electron capture detector.
Methods:With this method,ether was used as extraction solvent and trichloromethane was used as an internal standard. The whole blood sample was extracted with ether, and dehydrated by anhydrous sodium sulfate. Then the analytes were separated on HP-5 capillary column(30m×0.32mm×0.15μm)and detected byECD.The retention time was for qualitative analysis and the internal standard was for quantitation.
Results:The standard curves of TCE and TCOH showed significant linearity between 95.5μg/L-7640.0μg/L(r=0.9997)and 19.0μg/L-1520.0μg/L(r=0.9992). The average recovery was 95.5%-103.6%.The intra-day and inter-day precisions(RSD)were 2.5%-6.8%(n=6)and 1.6%-4.3%(n=6) respectively. The detect limit of TCE and TCOH were 2.10 μg/L and 0.56μg/L(S/N=3)respectively.The blood can be kept 7 days at-20℃ refrigerator without significantly loss.
Conclusion:This method is proved to be simple,practical and highly sensitive. It can satisfy the request for the determination of blood samples of humans exposed to TCE.
